MODEL PHOSPHOLIPID SURFACES VIA SELF ASSEMBLED MONOLAYER & SURFACE GRAFTING

通过自组装单层对磷脂表面进行建模

• 批准号: 6119944
• 负责人: S POLLACK
• 金额: $ 1.26万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-09-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119944
• 关键词: bioengineering /biomedical engineering; biological products; biomaterials; biomedical resource; lipids; model design /development; technology /technique

The study tests the hypothesis that a "controlled" phospholipid surfaceconsisting of a substrate, polydimethylsiloxane polymer and a phosphatidylcholine ester can be assembled by use of sequential grafting chemistries. Olefin functional trihydroxy silanes are reacted with glass or silicon dioxide to provide a self-assembled monolayer of reactive vinyl groups. Gold surfaces and alkane thiols will also be utilized to form a vinyl-rich surface by self-assembly. Afterwards hydride functional polysiloxanes are grafted to the surface vinyl groups using soluble platinum catalysts. Finally, the residual hydride groups on the polysiloxane are reacted again by platinum-catalyzed hydrosylilation of olefin functional phosphatidylcholine esters to create the ultimate blood-contacting layer. At each stage, the surface elemental composition is determined by ESCA and SIMS spectroscopies.

这项研究验证了一个假设，即“受控”磷脂 由基底、聚二甲基硅氧烷聚合物和聚二甲基硅氧烷聚合物组成表面 磷脂酰胆碱酯可以通过使用顺序的 接枝化学 烯烃官能三羟基硅烷是 与玻璃或二氧化硅反应， 反应性乙烯基的单层。 金表面和烷硫醇 也将用于通过自组装形成富含乙烯基的表面。 然后将氢化物官能化的聚硅氧烷接枝到表面上 使用可溶性铂催化剂的乙烯基。 最后，剩余 聚硅氧烷上的氢化物基团再次反应， 铂催化的烯烃官能基的氢硅烷化 磷脂酰胆碱酯可实现终极血液接触 层. 在每个阶段，确定表面元素组成 通过ESCA和西姆斯光谱。