FLUORESCENCE IMAGING CELLULAR DISTRIBUTION OF CA & MEMBRANE PHASE

CA 细胞分布的荧光成像

• 批准号: 6121646
• 负责人: Weiming Yu
• 金额: $ 0.47万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121646
• 关键词: animal tissue; biomedical equipment development; biomedical resource; human tissue; lipids; technology /technique

1. Membrane phase was monitored using the membrane probe Laurdan. Excitation of the laurdan-doped T-cells was accomplished using two-photon excitation and scanning across the T-cell to develop a complete 3-dimensional cellular map of membrane phase information. 2. Cellular free Ca- concentrations were monitored using the calicurn dye fura 2. Preliminary results on calcium standards have been performed with success. Future work includes 3-dimensional scanning of fura-containing T-cells and reconstruction of a 3D , free Ca concentration map of the cell.

1. 使用膜探针 Laurdan 监测膜相。 使用 laurdan 掺杂的 T 细胞进行激发 双光子激发和扫描 T 细胞以开发 膜相信息的完整 3 维细胞图。 2. 使用 calicurn 染料监测细胞游离 Ca 浓度 Fura 2. 钙标准品的初步结果已完成 成功了。 未来的工作包括 3 维扫描 含fura的T细胞和3D游离Ca的重建 细胞浓度图。