FLUORESCENCE IMAGING CELLULAR DISTRIBUTION OF CA & MEMBRANE PHASE

1. 使用膜探针Laurdan监测膜相。 月桂醇掺杂的T细胞的激发是使用 双光子激发和扫描整个T细胞， 完整的膜相位信息的三维细胞图。 2. 细胞内游离钙浓度用钙染法监测， fura 2. 钙标准品的初步结果已经完成 成功地 未来的工作包括三维扫描 含Fura的T细胞和3D游离Ca 细胞的浓度分布图。

1. Membrane phase was monitored using the membrane probe Laurdan. Excitation of the laurdan-doped T-cells was accomplished using two-photon excitation and scanning across the T-cell to develop a complete 3-dimensional cellular map of membrane phase information. 2. Cellular free Ca- concentrations were monitored using the calicurn dye fura 2. Preliminary results on calcium standards have been performed with success. Future work includes 3-dimensional scanning of fura-containing T-cells and reconstruction of a 3D , free Ca concentration map of the cell.