课题基金 / 基金详情

FLUORESCENCE IMAGING CELLULAR DISTRIBUTION OF CA & MEMBRANE PHASE

FLUORESCENCE IMAGING CELLULAR DISTRIBUTION OF CA & MEMBRANE PHASE
CA 细胞分布的荧光成像
批准号：
6121646
负责人：
Weiming Yu
金额：
$0.47万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-08-01 至 1999-07-31
来源：
https://reporter.nih.gov/project-details/6121646
关键词：
animal tissue biomedical equipment development biomedical resource human tissue lipids technology /technique

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英文摘要
1. Membrane phase was monitored using the membrane probe Laurdan. Excitation of the laurdan-doped T-cells was accomplished using two-photon excitation and scanning across the T-cell to develop a complete 3-dimensional cellular map of membrane phase information. 2. Cellular free Ca- concentrations were monitored using the calicurn dye fura 2. Preliminary results on calcium standards have been performed with success. Future work includes 3-dimensional scanning of fura-containing T-cells and reconstruction of a 3D , free Ca concentration map of the cell.
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