POLARIZATION OF FITC BSA PROBES

流式细胞仪荧光偏振Q FITC，BSA(x=5，10， 或20)和FITC-聚-D-酪氨酸探针在混合微噬菌体中显示 与时间和抗原浓度相关的某种趋势 以确定BSA探针的表位密度。为了研究这一趋势是否 由于在体外细胞内抗原的还原和展开 测量了相同探针的偏振度。还原和展开 FITC-BSA的结果显示，由于开放 BSA分子和更大的运动。类似的效果也观察到了 FITC-聚D-赖氨酸。

Flow cytometric fluorescence polarization q FITC, BSA (x = 5, 10, or 20) and FITC-poly-D-tykine probes in mixing microphage showed a certain trend with time and concentration of antigen that correlated to epitope density of the BSA probes. To study whether this trend was due to reduction and unfolding of the antigen inside the cell in-vitro polarigation of the same probes was measured. Reduction and unfolding of FITC-BSA showed a decrease in polarization due to opening up of the BSA molecule and greater motion. A similar effect was observed with FITC-poly-D-lysine.