SUBCELL STRUCT OF PHAEOCYSTIS & ITS RELATIONSHIP TO ITS ABSORPTION PROPERTIES

海洋颜色遥感的出现为我们提供了 有能力对时间进行概括性观察的海洋学家 以及全球范围内浮游植物色素的空间变异性。 通过使用生物光学算法，初级生产总值 通过了解色素的生物量可以估计出 叶绿素比吸收系数、量子效率和 入射辐照度。最近的几项研究表明，细胞 最终影响光学特性的特性 浮游植物随温度、光照和营养物质的变化是可预测的 限制。我建议测试一个生物光学模型的准确性 格陵兰海在裸子植物单一特有的水华期间， 棕囊藻。模型将根据以下结果进行参数化 温光限制条件下棕囊藻的培养 在营养充足的条件下。模型输出将与 来自CEAREX(协调东部)的现有初级生产数据 北极实验)乘船前往格陵兰海。对 模型将有助于更好地理解 生物光学模型的参数化将最终预测 利用海洋颜色资料研究棕囊藻水华的初级生产 卫星。实际的亚细胞吸收是在 叶绿体类囊体光通道的长度是 由细胞内部细胞器的分布决定的。在一个 之前使用透射式电子显微镜的研究结果 研究表明，类囊体堆积与光适应和 在14岁和20岁时，类囊体的堆积变化了近2倍 2595摩尔量子m-2S-1。我提议用电子显微镜 体层摄影术，以检查如何内部组织 棕囊藻的单细胞影响a*ph(？)然后呢？在……下面 温度限制。电子显微镜断层图像的使用将 给我们关于单个细胞的亚细胞组织的洞察 棕囊藻，这将有助于我们理解整体吸收 属性。已经进行了几次断层重建。 这些断层重建将被用来检查 叶绿体结构与吸收的关系 用蒙特卡罗模拟研究棕囊藻的性质。

The advent of Ocean Color remote sensing has provided oceanographers with the ability to observe synoptically the temporal and spatial variability of phytoplankton pigments on a global scale. Through the use of bio-optical algorithms, gross primary production estimates can be estimated from pigment biomass by knowing the chlorophyll-specific absorption coefficient, quantum yield and incident irradiance. Several recent studies have shown that cellular characteristics which ultimately affect the optical properties of phytoplankton vary predictably with temperature, light, and nutrient limitation. I propose to test the accuracy of a bio-optical model in the Greenland Sea during a mono-specific bloom of the prymnesiophyte, Phaeocystis. The model will be parameterized based on results from culture of Phaeocystis grown under temperature and light limitation uder nutrient-replete conditions. Model output will be compared to existing primary production data from the CEAREX (Coordi nated Eastern Arctic Experiment) cruise to the Greenland Sea. Refinements to the model will contribute to a better understanding of the parameterization of bio-optical models that will ultimately predict primary production of Phaeocystis blooms using data from Ocean Color Satellites. Actual subcellular absorption is accomplished in the chloroplast thylakoids and the length of the pathway of light is determined by the distribution of internal cellular organelles. In a previous study using transmission electron microscopy, results have shown that thylakoid stacking is related to photoadaptatiion and showed a near 2 fold change in thylakoid stacking when grown at 14 and 259 5mol quanta m-2S-1. I proposed to use electron microscope tomograms in order to examine how the internal organization of the single cells of Phaeocystis affects the a*ph (?? and ? under temperature limitation. The use of electron microscope tomograms will give us insight on subcellular organization of single cells of Phaeocystis which will help us to understand bulk absorption properties. Several tomographic reconstructions have been performed. These tomographic reconstructions will be used to examine the relationship between chloroplast structure and the absorption properties of Phae ocystis using Monte Carlo simulations.