SEAWATER SYSTEM RENOVATION FOR MARINE PHYSIOLOGY LAB

海洋生理实验室海水系统改造

• 批准号: 2609729
• 负责人: Barbara Kent
• 金额: $ 17.5万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2609729
• 关键词: animal care; animal colony; biomedical equipment purchase; biomedical resource; building /facility design /renovation; marine biology; saltwater environment

The long range goal of this animal resource project is to enhance the ability of the Mount Desert Island Biological Laboratory (MDIBL) to provide marine species to investigators for use in basic biomedical and toxicological research. MDIBL is an international research center where visiting investigators make use of unique marine species to investigate the molecular mechanisms that underlie the basic function of organs such as the kidney, the liver, the brain, the heart and the ye. MDIBL is currently the year round home for one research program in Developmental Biology and a corporate laboratory, that is collaborating in the development of facility for rearing fish embryos and transgenic animals. The ability of MDIBL to provide marine animals such as the shark, skate, flounder, sculpin, killifish, crabs, sea urchins, sand dollars, and snails is entirely dependent on the continuous function of a seawater system which takes in seawater from Frenchman Bay and distributes it to individual laboratories and central hold facilities. The current seawater system is the result of several decades of gradual evolution, ad hoc repairs and alterations. As a consequence the current system is highly compromised by inadequate pumping and suboptimal distribution as well as aging components that have a high failure rate and cannot be easily maintained. Frequent failure of the system leads to the loss of specimens or maintenance in suboptimal conditions thus impairing the ability of the Lab to supply animals for research. The specific aims are 1) to upgrade the seawater intake system by installing high capacity, self priming pumps equipped with duplicate intake lines to permit periodic preventative maintenance; and to improve the safety and accessibility of the pumping stating; 2) to completely redesign and renovate the seawater distribution system to assure adequate flow rates to all laboratories and holding areas, and allow for duplicate lines that facilitate cleaning and prevent freezing of the lines in winter; 3) to renovate fish holding areas to increase the capacity and the quality control of the seawater and permit expanded seasonal and year-round use; and 4) to implement an expanded animal health program that will include increased monitoring of animal holding tans and veterinary supervision necessary for holding animals for long periods. The proposed renovation will enhance the ability of MDIBL to provide marine specimens to visiting investigators, permit expanded year- round use of the animal facility and provide the foundation for reestablishing a core facility for generating and maintaining transgenic mice.

这个动物资源项目的长期目标是提高 荒岛山生物实验室（MDIBL） 向调查人员提供海洋物种，用于基础生物医学， 毒理学研究MDIBL是一个国际研究中心， 访问调查员利用独特的海洋物种进行调查 器官基本功能的分子机制， 如肾、肝、脑、心和叶。MDIBL是 目前，一个研究项目在发展中国家的全年家庭 生物学和一个公司实验室， 发展培育鱼类胚胎和转基因动物的设施。 MDIBL提供海洋动物，如鲨鱼，溜冰鞋， 比目鱼、杜父鱼、鳉鱼、螃蟹、海胆、沙元和蜗牛 完全依赖于海水系统的连续功能 从法国人湾抽取海水， 个别实验室和中央保存设施。目前的海水 制度是几十年来逐步演变的结果，特别是 维修和改建。因此，目前的制度高度 由于泵送不足和次优分配， 故障率高且不易更换的老化组件 树立政治意识系统的频繁故障导致试样的丢失 或在次优条件下维持，从而损害 实验室为研究提供动物。具体目标是：1）提升 安装高容量自吸泵的海水吸入系统 配备双进气管，以允许定期预防 以及改善抽水的安全性和可及性。 指出：2）全面重新设计和改造海水分配 系统，以确保所有实验室和保持足够的流速 区域，并允许重复线，便于清洁和防止 冬季冻结鱼线; 3）修复养鱼区， 增加海水的容量和质量控制， 扩大季节性和全年使用;以及4）实施扩大的 动物健康计划，将包括增加对动物的监测 保持制革和兽医监督必要的持有动物， 很长时间拟议的翻修将提高MDIBL的能力， 向来访的调查人员提供海洋标本，允许延长一年- 动物设施的全面使用，并为 重建一个核心设施， 小鼠