NATURAL ANTIBODY AGAINST GROUP B STREPTOCCOUS IN MICE

小鼠 B 组链球菌天然抗体

• 批准号: 6226245
• 负责人: PETER BUTKO
• 金额: $ 14.27万
• 依托单位: UNIVERSITY OF SOUTHERN MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6226245
• 关键词: Streptococcus agalactiae; antibacterial antibody; bacteria infection mechanism; bacterial antigens; bactericidal immunity; enzyme linked immunosorbent assay; genetic strain; humoral immunity; immunoglobulin M; laboratory mouse

DESCRIPTION (Verbatim from Applicant's Abstract): Group B streptococcus (GBS, Streptococcus agalactiae) is the most common cause of neonatal sepsis and meningitis in the USA. A humoral factor has been discovered in normal mouse serum which protects the animals against GBS infection. It is hypothesized that the protective factor is natural IgM antibody. Two goals of the proposed research are: (i) To confirm that the antibody is of the IgM type and that it is protective against experimental GBS infections in vivo; (ii) to identify the antigenic target for this antibody on the bacterial surface. To these aims, several clinical isolates and mutant strains of GBS, in combination with in vitro enzyme-linked immunosorbent assays (ELISA) and in vivo mouse protection experiments will be utilized. Results obtained with mice as animal model may open a new avenue of investigation into possible role of natural antibodies in human immunity to GBS infection and bacterial infection in general, with possible implications for immunotherapy. The results also may provide impetus to the research on the antigenic targets on GBS, possibly other than the capsular polysaccharide, which would have implications for current GBS vaccine efforts. A non-scientific goal of this project is to increase the intensity of health-related undergraduate research and teaching in a mid-size rural university with no medical school and to increase diversity in the work force in the academic research and medicine.

描述(逐字摘自申请人摘要)：B组链球菌(GBS， 无乳链球菌)是新生儿败血症最常见的原因， 美国的脑膜炎。在正常小鼠身上发现了一种体液因子 保护动物免受GBS感染的血清。据推测， 保护性因素是天然的IgM抗体。建议的两个目标 研究是：(I)确认抗体是IgM型，并确认 在体内对实验性的GBS感染具有保护作用；(Ii)确定 这种抗体在细菌表面的抗原靶标。为了实现这些目标， 几株GBS临床分离株和突变株与In 体外酶联免疫吸附试验(EL ISA)及其体内保护作用 将利用实验。以小鼠为动物模型获得的结果可能 开辟了一条研究天然抗体在人类免疫缺陷病毒中可能作用的新途径 人类对GBS感染和一般细菌感染的免疫力， 可能对免疫治疗的影响。这一结果也可能提供动力 对GBS上抗原靶标的研究，可能不是 壳多糖，这将对目前的GBS疫苗产生影响 努力。这个项目的一个非科学目标是增加 中等规模农村卫生相关本科科研与教学 没有医学院的大学，并增加劳动力的多样性 在学术研究和医学方面。