THE J-C INTRON: A MUTATION INDICATOR IN BOVINE V GENES
J-C 内含子:牛 V 基因中的突变指标
基本信息
- 批准号:6225242
- 负责人:
- 金额:$ 14.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2001
- 资助国家:美国
- 起止时间:2001-04-01 至 2004-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (Adapted from the Applicant's Abstract): Rearrangement and somatic
mutation contribute to primary antibody repertoire diversification in cattle.
Untemplated somatic mutation, gene conversion, or a combination of both, is
thought to be the major generator of repertoire diversification in the Bovidae.
However, much of the repertoire of heavy and light chain V genes in cattle and
sheep remains unknown. Thus, it is difficult to determine, definitively,
whether particular variations in expressed antibody genes are due to mutation
or rearrangement. It is also difficult to discriminate between gene conversion
and untemplated somatic point mutation. The proposed studies entail experiments
that will use variation in J-C intron sequences to resolve these issues. The
following specific aims were listed: 1) Use of the J-C intron as a diagnostic
indicator of the mutational mechanisms used in bovine lambda light chain
diversification. 2) Use of the bovine heavy chain J-C intron as a neutral
indicator of bovine heavy chain mutational diversification. 3) Use of the J-C
intron as a diagnostic indicator of the mutational mechanism employed in bovine
heavy chain mutational diversification. Examination of the J-C intron provides
both a neutral indicator of mutation and a way a to discriminate between gene
conversion (templated mutation) and somatic mutation (untemplated mutation).
Characterization of light and heavy chain J-C introns and demonstration of
their utility as "mutometers" for detecting the occurrence and mechanism of
mutation in bovine antibody genes will establish a useful tool for analysis of
variation in bovine Ig genes. The applicant proposes that this approach may
provide a convenient assay for future study of somatic mutation of bovine
immunoglobulin genes in organ and cell culture.
描述(改编自申请人摘要):重排和体细胞
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Richard A Goldsby其他文献
Richard A Goldsby的其他文献
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{{ truncateString('Richard A Goldsby', 18)}}的其他基金
Synergistic combinations of chimeric anti-SEB: Engineering anti-toxins
嵌合抗SEB的协同组合:工程抗毒素
- 批准号:
7371194 - 财政年份:2008
- 资助金额:
$ 14.27万 - 项目类别:
UNUSUAL PATHS AND SITES OF V REGION DIVERSIFICATION
V区多元化的不寻常路径和地点
- 批准号:
2604509 - 财政年份:1998
- 资助金额:
$ 14.27万 - 项目类别:
TIMING ONSET OF B CELL REPERTOIRE GENERATION AND EXPORT
B 细胞库生成和输出的开始时间
- 批准号:
2437503 - 财政年份:1995
- 资助金额:
$ 14.27万 - 项目类别:
TIMING ONSET OF B CELL REPERTOIRE GENERATION AND EXPORT
B 细胞库生成和输出的开始时间
- 批准号:
2074575 - 财政年份:1995
- 资助金额:
$ 14.27万 - 项目类别:
POLYCLONAL LIBRARY--RESOURCES FOR ANTIBODIES & CATALYSIS
多克隆文库--抗体资源
- 批准号:
3438708 - 财政年份:1989
- 资助金额:
$ 14.27万 - 项目类别:
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