STUDIES OF THE V-<I>ETS</I> ETS1 AND FLI1 TRANSCRIPTION FACTORS IN HEMATOPOIETIC

ETS家族的转录因子已被证明与细胞增殖和各种肿瘤转化事件相关，特别是涉及红系谱系的那些。我们一直感兴趣的ETS-1和FLI-1基因，ETS家族的转录因子的两个成员，在造血肿瘤和正常分化的作用。ETS家族的成员已被证明参与体内红细胞和巨核细胞分化的各个方面。我们的前期工作表明，FLI-1在某些多能造血细胞系中激活巨核细胞分化过程中正常表达的基因，同时阻断与红系分化相关的基因的表达。我们已经扩展了我们的机制，MKK/MAPK和FLI-1都可以激活巨核细胞标志物在K562和HEL细胞的表达的研究。我们的数据表明，这些影响可能涉及两个独立的途径，外源性FLI-1的存在改变了MKK/MAPK激活和抑制的影响。此外，这些研究表明，gpII_B和gpIII_A蛋白水平的调节可能受到这些途径的不同影响。 - 红系，ETS 1，FLI 1，HEL，造血，K，2，巨核细胞，转录因子，-非人类受试者或人类组织

The ETS family of transcription factors have been shown to be associated cell proliferation and a variety on neoplastic transforming events, especially those involving the erythroid lineage. We have been interested in the role of the ETS-1 and FLI-1 genes, two members of the ETS family of transcription factors, in hematopoeitic neoplasia and normal differentiation. Members of the ETS family have been shown to be involved in aspects of both erythroid and megakaryocytic differentiation in vivo. Our previous work had indicated that FLI-1 acted positively in certain pluripotent human hematopoeitic cell lines to activate genes normally expressed during megakaryocytic differentiaton, while blocking expression of genes associated with erythroid differentiation. We have extended our studies of the mechanisms by which MKK/MAPK and FLI-1 both can activate the expression of megakaryocytic markers in K562 and HEL cells. Our data indicate that these effects may involve two separate pathways, and that the presence of exogenous FLI-1 alters the effects of MKK/MAPK activation and inhibition. In addition these studies suggest that the regulation of the gpII_B and gpIII_A protein levels may be differentially affected by these pathways. - erythroid, ETS1, FLI1, HEL, hematopoiesis, K, 2, megakaryocyte, transcription factor, - Neither Human Subjects nor Human Tissues