BIOLOGICAL CONSEQUENCES OF PROTEIN OXIDATION

蛋白质氧化的生物学后果

• 批准号: 6293781
• 负责人: EMILY B SHACTER
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6293781
• 关键词: carbohydrate metabolism; carbonyl compound; cytotoxicity; fibrinogen; glycoproteins; human tissue; hydrogen peroxide; lipid metabolism; oncoproteins; oxidation; oxidative stress; protease inhibitor; protein metabolism; proteolysis; western blottings

For the last year, research for this project number was carried out under new project number Z01 BN02020-01 LIM (Oxidants and cell death). Hence, we have no new results for this project. However, we are now initiating a new series of studies designed to determine how protein oxidation might play a role in cell death. Specifically, experiments will be carried out to assess mitochondrial protein oxidation during chemotherapy induced apoptosis and H2O2 induced cell death. Mitochondrial function is central to the apoptotic process and there are several key mitochondrial proteins which are particularly susceptible to oxidative modification. We will look to see whether these proteins are modified during cell death and will determine whether this oxidation has any effect on cell death pathways. Other subcellular fractions will also be tested (e.g., plasma membrane, nuclear, and cytosolic fractions). The target cells for these studies will be human lymphoma and breast cancer cells.

去年，该项目编号的研究在新项目编号Z01 BN02020-01 LIM（氧化剂和细胞死亡）下进行。因此，我们对这个项目没有新的结果。然而，我们现在正在启动一系列新的研究，旨在确定蛋白质氧化如何在细胞死亡中发挥作用。具体来说，我们将开展实验来评估化疗诱导的细胞凋亡和H2O2诱导的细胞死亡过程中线粒体蛋白氧化的影响。线粒体功能是凋亡过程的核心，有几个关键的线粒体蛋白特别容易受到氧化修饰。我们将观察这些蛋白质在细胞死亡过程中是否被修饰，并确定这种氧化是否对细胞死亡途径有任何影响。其他亚细胞组分也将被检测（例如，质膜、核和细胞质组分）。这些研究的靶细胞将是人类淋巴瘤和乳腺癌细胞。