Alternative Photosensitization Assay in the Mouse

描述（由申请人提供）：本提案，"备选方案" 小鼠光敏化试验（Photo-LLNA）"是一种替代动物 用于鉴定具有免疫毒性的物质的试验。一直 形成了行业需求和政府授权， 毒性试验指的是，减少或取代使用动物模型。由于 越来越多的关注光过敏（光敏化）性能 化学品，有越来越多的需要快速，成本效益的测定， 不仅可以预测敏化作用，而且可以预测光敏作用。当地 淋巴结试验已被证明是一个有用的，敏感的模型，用于检测 致敏剂。本提案的目的是证明以下做法的可行性： 采用LLNA，结合紫外线照射，作为测试系统，以确定 潜在的光敏剂或光刺激剂。很少有论文发表在 使用该模型的文献，因此这是一个相对新颖的应用， 这个测试系统。小鼠模型的选择有几个优点， 豚鼠试验，包括使用已知的预测机制试验 系统，这是快速，具有成本效益，并尽量减少痛苦和痛苦， 动物Photo-LLNA检测将提供灵敏的检测系统，同时：（1） （2）遵守《动物福利法》，直接解决3R问题; 提高了比较时产生的数据的鉴别能力和质量 （3）大大降低与使用相关的成本 用于光敏化研究。 拟定商业应用： MB Research将为制药、生物技术、化妆品和化学行业的成员提供替代光敏化测定服务。对我们客户的调查表明，对快速和具有成本效益的光敏化测试以及提供定性和定量信息的基于机械的测试的需求日益增加。 此外，我们认为流式细胞术在毒性筛选领域未得到充分利用，该技术可用于在我们公司开发其他商业上可行的体内和体外毒性试验。

DESCRIPTION (provided by applicant): This proposal, "Alternative Photosensitization Assay in the Mouse (Photo-LLNA)," is an alternative animal test designed to identify substances with immunotoxic properties. There has evolved an industry demand and a governmental mandate to develop alternative toxicity tests that refer, reduce, or replace the use of animal models. Due to increasing concerns over the photoallergic (photosensitizing) properties of chemicals, there is an increasing need for rapid, cost-effective assays, which can predict not only sensitization, but also photosensitization. The Local Lymph Node Assay has proven to be a useful, sensitive model for detecting sensitizers. The purpose of this proposal is to demonstrate the feasibility of employing the LLNA, combined with UV irradiation, as a test system to identify potential photosensitizers or photoirritants. Few papers have been published in the literature using this model, thus this is a relatively novel application of this test system. The choice of a mouse model has several advantages over guinea pig tests, including the use of a known predictive mechanistic assay system, which is rapid, cost-effective, and minimizes pain and distress to animals. The Photo-LLNA Assay will provide a sensitive test system while; (1) complying with the Animal Welfare Act by directly addressing the 3Rs; (2) increasing the discriminating power and quality of data generated when compared to existing methods; and (3) greatly reducing the costs associated with the use of animals for photosensitization studies. PROPOSED COMMERCIAL APPLICATION: MB Research will offer the service of an alternative photosensitization assay to members of the pharmaceutical, biotech, cosmetic, and chemical industry. A survey of our clients indicates there is an increassing demand for rapid and cost-effective photosensitization tests, as well as for mechanistic-based tests, which provide both qualitative and quantitative information. In addition, we believe that flow cytometry is underutilized in the toxicity-screening field and that this technology can be used to develop other commercially viable in vivo and vitro toxicity test at our company.