Alternative Photosensitization Assay in the Mouse

描述(由申请人提供)：本建议书，“替代方案” 小鼠的光敏化试验(Photo-LLNA)，是一种替代动物 用来鉴定具有免疫毒性的物质的试验。那里有 形成了行业需求和政府命令，以开发替代方案 参考、减少或取代动物模型使用的毒性测试。由于 对光过敏(光敏)特性的日益关注 对于化学品来说，对快速、经济有效的分析的需求越来越大，这 不仅可以预测敏化，还可以预测光敏化。地方性的 淋巴结节检测已被证明是一种有用的、敏感的检测模型 感光剂。这项建议的目的是证明……的可行性。 使用LLNA，结合紫外线照射作为测试系统，以识别 潜在的光敏剂或光刺激物。很少有论文发表在 使用这种模型的文献，因此这是一种相对新颖的应用 这个测试系统。与选择鼠标型号相比，选择鼠标型号有几个优点 豚鼠试验，包括使用一种已知的预测机制分析 系统，该系统快速、经济高效，并将痛苦和痛苦降至最低 动物。照片-LLNA分析将提供一个敏感的测试系统；(1) 遵守动物福利法，直接处理3R；(2) 提高比较时生成的数据的辨别力和质量 现有方法；以及(3)大大降低与使用相关的成本 用于光敏研究的动物。 建议的商业应用： MB Research将为制药、生物技术、化妆品和化工行业的成员提供替代光敏测试服务。对我们客户的一项调查表明，对快速且经济有效的光敏测试以及提供定性和定量信息的机械测试的需求日益增长。此外，我们认为，流式细胞术在毒性筛选领域的应用还不够充分，这项技术可以用于我们公司其他商业上可行的体内和体外毒性测试。

DESCRIPTION (provided by applicant): This proposal, "Alternative Photosensitization Assay in the Mouse (Photo-LLNA)," is an alternative animal test designed to identify substances with immunotoxic properties. There has evolved an industry demand and a governmental mandate to develop alternative toxicity tests that refer, reduce, or replace the use of animal models. Due to increasing concerns over the photoallergic (photosensitizing) properties of chemicals, there is an increasing need for rapid, cost-effective assays, which can predict not only sensitization, but also photosensitization. The Local Lymph Node Assay has proven to be a useful, sensitive model for detecting sensitizers. The purpose of this proposal is to demonstrate the feasibility of employing the LLNA, combined with UV irradiation, as a test system to identify potential photosensitizers or photoirritants. Few papers have been published in the literature using this model, thus this is a relatively novel application of this test system. The choice of a mouse model has several advantages over guinea pig tests, including the use of a known predictive mechanistic assay system, which is rapid, cost-effective, and minimizes pain and distress to animals. The Photo-LLNA Assay will provide a sensitive test system while; (1) complying with the Animal Welfare Act by directly addressing the 3Rs; (2) increasing the discriminating power and quality of data generated when compared to existing methods; and (3) greatly reducing the costs associated with the use of animals for photosensitization studies. PROPOSED COMMERCIAL APPLICATION: MB Research will offer the service of an alternative photosensitization assay to members of the pharmaceutical, biotech, cosmetic, and chemical industry. A survey of our clients indicates there is an increassing demand for rapid and cost-effective photosensitization tests, as well as for mechanistic-based tests, which provide both qualitative and quantitative information. In addition, we believe that flow cytometry is underutilized in the toxicity-screening field and that this technology can be used to develop other commercially viable in vivo and vitro toxicity test at our company.