STAPHYLOCOCCUS AND METHICILLIN RESISTANCE ASSAY

描述(申请人提供)：Nariphere，Inc.目前正在开发 一种新的超灵敏、高选择性的核酸序列检测方法 以芯片为基础的形式的核酸。该方法是基于杂交法 靶向纳米寡核苷酸固定化的靶向多核苷酸 在玻璃表面上，然后是专有的“显影”程序，允许 一种是以量热的方式探测目标。操作的简单性， 低成本和快速的分析使其潜在地非常有吸引力 检测各种传染病和遗传病。此外，ITS 基于芯片的格式使其易于适应多路复用和敏感度 指出了检测处理过的基因组目标的有趣的可能性 不含聚合酶链式反应(PCR)。拟议研究的目标是 建立一种快速、同步检测mecA基因的方法 携带、凝固酶基因存在和多个16S核糖体RNA基因 序列。这些基因靶点将决定物种识别和 7株临床相关葡萄球菌的甲氧西林耐药状况。 建议的商业应用： 一种快速、同时检测mecA基因携带者的方法， 凝固酶基因的存在，以及多个16S核糖体RNA基因序列。 基因靶标将决定菌种鉴定和甲氧西林耐药性 七种临床相关葡萄球菌的状况有了很大的改善 在病人护理方面。

DESCRIPTION (provided by applicant): Nariosphere, Inc. is currently developing a new ultrasensitive and selective method for detecting nucleotide sequences in nucleic acids via a chip-based format. The method is based on hybridization of a target polynucleotide of a target to nanoparticleoligonucleotides immobilized on a glass surface followed by a proprietary "developing" procedure that allows one to detect targets in a calorimetric fashion. The simplicity of operation, low cost, and speed of the assay make it potentially very attractive for detecting a wide variety of infectious and genetic diseases. Moreover, its chip-based format makes it easily adaptable to multiplexing and sensitivity points to the intriguing possibility of detecting processed genomic targets without PCR (Polymerase Chain Reaction). The goal of the proposed research is to develop a rapid, simultaneous assay permitting determination of mecA gene carriage, coagulase gene presence, and multiple 16S ribosomal RNA gene sequences. These gene targets will determine species identification and methicillin resistance status for seven clinically relevant Staphylococci. PROPOSED COMMERCIAL APPLICATION: A rapid, simultaneous assay permitting determination of mecA gene carriage, coagulase gene presence, and multiple 16S ribosomal RNA gene sequences. Gene targets will determination species identification and methicillin resistance status of seven clinical relevant Staphylococci offering a tremendous improvement in patient care.