STAPHYLOCOCCUS AND METHICILLIN RESISTANCE ASSAY

描述（由申请人提供）：Nariosphere，Inc.目前正在开发 一种新的超灵敏和选择性的检测核苷酸序列的方法， 核酸通过基于芯片的格式。该方法是基于杂交的 将靶的靶多核苷酸固定到纳米颗粒寡核苷酸 在玻璃表面上，然后进行专有的“显影”程序， 一个是以量热的方式探测目标操作简单， 低成本和测定速度使其潜在地非常吸引人， 检测各种传染病和遗传病。而且其 基于芯片的格式使其易于适应多路复用和灵敏度 指出了一种有趣的可能性， PCR（Polymerase Chain Reaction）拟议研究的目标是 开发一种快速、同时测定mecA基因的方法， 携带、凝固酶基因存在和多个16S核糖体RNA基因 序列的这些基因靶点将决定物种鉴定， 7种临床相关葡萄球菌的甲氧西林耐药状态。 拟定商业应用： 一种快速、同时测定mecA基因携带的方法， 凝固酶基因的存在和多个16S核糖体RNA基因序列。 基因靶标将决定物种鉴定和甲氧西林耐药性 七种临床相关葡萄球菌的状态提供了巨大的改善 在病人护理中。

DESCRIPTION (provided by applicant): Nariosphere, Inc. is currently developing a new ultrasensitive and selective method for detecting nucleotide sequences in nucleic acids via a chip-based format. The method is based on hybridization of a target polynucleotide of a target to nanoparticleoligonucleotides immobilized on a glass surface followed by a proprietary "developing" procedure that allows one to detect targets in a calorimetric fashion. The simplicity of operation, low cost, and speed of the assay make it potentially very attractive for detecting a wide variety of infectious and genetic diseases. Moreover, its chip-based format makes it easily adaptable to multiplexing and sensitivity points to the intriguing possibility of detecting processed genomic targets without PCR (Polymerase Chain Reaction). The goal of the proposed research is to develop a rapid, simultaneous assay permitting determination of mecA gene carriage, coagulase gene presence, and multiple 16S ribosomal RNA gene sequences. These gene targets will determine species identification and methicillin resistance status for seven clinically relevant Staphylococci. PROPOSED COMMERCIAL APPLICATION: A rapid, simultaneous assay permitting determination of mecA gene carriage, coagulase gene presence, and multiple 16S ribosomal RNA gene sequences. Gene targets will determination species identification and methicillin resistance status of seven clinical relevant Staphylococci offering a tremendous improvement in patient care.