Zinc Sensors for Neurology and Neuroscience

描述：我们将为神经学构建可植入的光纤锌传感器 和神经科学这些传感器将利用专有的，遗传的 工程蛋白质（光纤），以提供实时监测， 颅内锌离子。这些传感器既用于基础研究， 含锌神经元和急诊室/ICU中的急性监测 脑损伤患者 监测颅内Zn 2+在临床上是重要的，因为Zn 2 +/-离子是 兴奋性神经元损伤的一个原因。头部创伤，局部缺血， 和癫痫发作，仅仅螯合细胞外锌就能大大减少 损伤后出现退行性变化的神经元数量。我们的临床 锌传感器被设计为通过脑室造口术插入，在 传统颅内压探头。在急性脑损伤患者中， Zn 2+水平升高会提醒外科、ER或ICU工作人员进行干预 积极使用锌释放阻滞剂，锌螯合剂，锌通道阻滞剂， 或其它针对锌介导的神经毒性的疗法。 对于基础神经科学实验室，我们的光纤传感器将允许 实时监测锌的突触释放到细胞外 环境。正如监测变送器释放的较旧方法（伏安法， 微透析）彻底改变了我们对经典递质的理解， 对Zn 2+释放的光学监测也可以帮助我们理解 锌调节的突触功能 拟定商业应用：不可用

DESCRIPTION: We will build implantable, fiberoptic, zinc sensors for neurology and neuroscience. These sensors will utilize proprietary, genetically engineered proteins (on optical fibers) to provide real-time monitoring of intracranial Zn2+. The sensors are needed both for basic research on zinc-containing neurons and in the emergency room/ICU for monitoring acutely brain-injured patients. Monitoring intracranial Zn2+ is clinically important because the Zn2+/- ion is a contributing cause of excitotoxic neuron injury. In head trauma, ischemia, and seizures, merely chelating extracellular zinc substantially reduces the number of neurons showing degenerative changes after the insult. Our clinical zinc sensor is designed to be inserted through a ventriculostomy, beside the traditional intracranial pressure probe. In acutely brain-injured patients, rising levels of Zn2+ would alert surgical, ER or ICU staff to intervene aggressively with zinc-release blockers, zinc chelators, zinc channel blockers, or other therapies against zinc-mediated neurotoxicity. For the basic neuroscience laboratory, our fiberoptic sensors will allow the real-time monitoring of the synaptic release of zinc into the extracellular milieu. Just as older methods for monitoring transmitter release (voltametry, microdialysis) revolutionized our understanding of classical transmitters, so too can optical monitoring of Zn2+ release inform our understanding of zinc-modulated synaptic function. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE