FLOW (CYTOMETRY)-BASED UNSCHEDULED DNA SYNTHESIS ASSAY

描述：提出了一种改进的遗传毒性检测方法。 该测试将基于非计划DNA合成测试，该测试将测量 DNA损伤修复所致的核苷酸掺入。该提案旨在 修改检测方法，使用非放射性示踪剂和流式细胞术。这个 成功完成拟议的工作将使申请人能够 组织提供重要的新服务，以化学品、化妆品、 消费品和安全检测行业。 建议的商业应用： MB Research将对制药、生物技术、化妆品、农用化学品、化工和消费品行业的成员进行这种(内部)遗传毒性测试。我们的客户越来越多地要求快速、可靠和廉价的遗传毒性分析(参见Brayer、雅芳和珀杜制药公司的信函)，用于筛选大量化学物质。这种检测的结果将被用来确定哪些化学品应该被排除在产品开发过程之外，哪些试剂应该继续进行进一步的遗传毒性测试。

DESCRIPTION: The development of an improved assay for genotoxicity is proposed. The assay will be based on the Unschedule DNA Synthesis assay that measures nucleotide incorporation due to repair of DNA damage. The proposal seeks to modify the assay to use non-radioactive tracers and flow cytometry. The successful completion of the proposed work will enable the applicant organization to provide an important new service to the chemical, cosmetic, consumer products and safety-testing industries. PROPOSED COMMERCIAL APPLICATION: MB Research will conduct this assay of genotoxicity (in-house) for members of the pharmaceutical, biotech, cosmetic, agrochemical, chemical and consumer products industries. Our clients are increasingly requesting rapid, reliable and inexpensive assays of genotoxicity (see letters from Brayer, Avon, and Perdue Pharma) that would be used to screen large numbers of chemicals. The results of such an assay would be used to determine which chemicals should be excluded from the product development process and which agents should move on to further tests of genotoxicity.