FLOW (CYTOMETRY)-BASED UNSCHEDULED DNA SYNTHESIS ASSAY

基于流式细胞术的非计划 DNA 合成测定

• 批准号: 6292965
• 负责人: EDWARD J YURKOW
• 金额: $ 10万
• 依托单位: MB RESEARCH LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292965
• 关键词: DNA damage; DNA replication; chemical property; environmental contamination; flow cytometry; high throughput technology; laboratory mouse; laboratory rat; liver cells; pesticides; technology /technique development; toxicant screening

DESCRIPTION: The development of an improved assay for genotoxicity is proposed. The assay will be based on the Unschedule DNA Synthesis assay that measures nucleotide incorporation due to repair of DNA damage. The proposal seeks to modify the assay to use non-radioactive tracers and flow cytometry. The successful completion of the proposed work will enable the applicant organization to provide an important new service to the chemical, cosmetic, consumer products and safety-testing industries. PROPOSED COMMERCIAL APPLICATION: MB Research will conduct this assay of genotoxicity (in-house) for members of the pharmaceutical, biotech, cosmetic, agrochemical, chemical and consumer products industries. Our clients are increasingly requesting rapid, reliable and inexpensive assays of genotoxicity (see letters from Brayer, Avon, and Perdue Pharma) that would be used to screen large numbers of chemicals. The results of such an assay would be used to determine which chemicals should be excluded from the product development process and which agents should move on to further tests of genotoxicity.

描述：拟定开发一种改进的遗传毒性试验。 该试验将基于非计划DNA合成试验， 由于DNA损伤的修复而导致的核苷酸掺入。该提案旨在 修改测定法以使用非放射性示踪剂和流式细胞术。的 成功完成拟议的工作将使申请人 组织提供一个重要的新的服务，化学，化妆品， 消费品和安全测试行业。 拟定商业应用： MB Research将为制药、生物技术、化妆品、农用化学品、化学品和消费品行业的成员进行遗传毒性试验（内部）。我们的客户越来越多地要求快速，可靠和廉价的遗传毒性测定（见Brayer，Avon和Perdue Pharma的信件），用于筛选大量化学品。这种试验的结果将用于确定哪些化学品应排除在产品开发过程之外，哪些试剂应继续进行遗传毒性的进一步试验。