Transfection Array Technology to Study Gene Functions

描述(申请人提供)：人类基因组的测序是一个 重塑了生物学和医学的不朽成就。我们现在开始 理解DNA代码的含义的挑战，特别是理解 所有基因的功能，其中大约10万个在人类中。这项艰巨的任务 将通过一些高通量技术的发展来加速 就像DNA测序中使用的那些一样。提出了基因转导阵列技术 作为一个如此强大的工具，它可以同时将一个大的 进入靶细胞的基因数量。在转基因后，这些基因将被 在靶细胞中表达，细胞子集中的每个基因 预定义位置并可角色化。因此，这项技术允许 体内基因功能的高通量研究。在这个项目中专门 设计的适合于转基因的DNA阵列将被开发和 通过电穿孔的最佳条件，将进行了研究。它 预计转染阵列技术将提供一个急需的工具 对数千个新克隆的基因进行功能研究。 建议的商业应用： 拟议的研究将开发一种技术，使高通量研究成为可能 基因的功能。学术研究将需要对技术在哪里的理解 基因功能的研究将加深对生命和疾病的理解。制药业 工业也将需要这项技术，因为它是发现更多药物的关键 目标。

DESCRIPTION (provided by applicant): The sequencing of the human genome is a monumental achievement that has reshaped biology and medicine. We now begin the challenge to understand the meanings of the DNA codes, especially to understand the functions of all genes, about 100,000 of them in human. This daunting task will be accelerated by the development of some high throughput technologies like those used in DNA sequencing. Transfection Array Technology is proposed here as such a powerful tool, which could simultaneously transfecting a large number of genes into target cells. After transfection, these genes will be expressed in the target cells, each gene in a subset of the cells at a pre-defined position and can be characterized. Therefore, the technology allows high-throughput study of gene functions in vivo. In this project specially designed DNA arrays that are suitable for transfections will be developed and the optimal condition for transfection by electroporation will be examined. It is expected that Transfection Array Technology will provide a much-needed tool for the functional study of the thousands newly cloned genes. PROPOSED COMMERCIAL APPLICATION: The proposed research will develop a technology that enables high throughput study of gene functions. Academic research will demand the technology where the understanding of gene functions will deepen the understanding of life and disease. Pharmaceutical industry will also demand this technology because it is a key to the discovery of more drug targets.