Single Cell Screen for Enzyme Expression and Activity

描述(由申请人提供)：Devera利用其专有技术 基因库生成和基因进化技术提供强大的 生物催化解决方案覆盖广泛的化学工业。这个 用于酶发现和进化的技术需要筛选 高吞吐量格式的超大数量(6log10-9log10克隆)。 提高吞吐量和敏感度是提高 这些筛查的成功率。这个项目的目标是开发一种通用的 将酶活性与高表达水平相关联的技术 吞吐量和非侵入式方式。我们提出了一种新的方法来扩展 一系列可筛选的酶，并提高了敏感性和重复性 活动屏幕。我们预计筛查技术的这些进步将会增加 Devera为农业、制药和医疗行业生产新酶的能力 化学应用。这些酶将使高效的生化 对提高生产力和减少对环境的影响的反应 许多美国公司。 建议的商业应用：不可用

DESCRIPTION (provided by applicant): Diversa leverages its proprietary technology in gene library generation and gene evolution to provide robust bio-catalytic solutions across a broad spectrum of chemical industries. The techniques used for enzyme discovery and evolution necessitate the screening of extremely large numbers (6log10 - 9log10 clones) in a high throughput format. Improvements to throughput and sensitivity are key issues in increasing the success rate of these screens. The goal of this project is to develop a general technique to correlate enzyme activity and expression levels in a high throughput and non-intrusive manner. We propose a novel method to expand the range of screenable enzymes and enhance the sensitivity and reproducibility of activity screens. We expect these advances in screening technology to increase Diversa's capacity to produce new enzymes for agricultural, pharmaceutical and chemical applications. These enzymes will enable efficient biochemical reactions to enhance the productivity and reduce the environmental impact of many US companies. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE