CORE--BONE HISTOMORPHOMETRY

核心——骨组织形态学

• 批准号: 6201521
• 负责人: DAVID W. DEMPSTER
• 金额: $ 13.97万
• 依托单位: HELEN HAYES HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6201521
• 关键词: animal tissue; biomedical facility; bone density; bone development; histology; human tissue; light microscopy; magnetic resonance imaging; morphology; osteoporosis; scanning electron microscopy

This histomorphometry core has been in integral part of our SCOR program since its inception in 1987. In the present application, the core provides histomorphometric support for four of the five projects and for the feasibility project on assessment of 3-d structure. Over the course of the five years the core will prepare and analyze over 600 rat and mouse specimens and 200 human biopsies. Bone histomorphometry will be employed to characterize the skeletal phenotype of transgenic mice which lack the p53 tumor suppressor gene. Bone histomorphometry will be used extensively to explore the mechanism of the anabolic action of PTH and interaction between dietary calcium deficiency and PT administration. Bone histomorphometry will be employed to characterize the cellular and structural effects of parathyroidectomy in postmenopausal women with mild primary hyperparathyroidism. Bone histomorphometry will be used to explore the mechanism of the initial intense anabolic action of PTH in patients with osteoporosis. Structural variables, assessed by Micro-MRI, will be correlated with histomorphometric variables of both cancellous and cortical bone structure. This core will employ state of the art technology and will bring over 15 years of experience to the analysis of the samples and interpretation of the data.

这个组织形态学核心已经成为我们SCOR计划的组成部分 自1987年成立以来。在本申请中，芯部提供 为五个项目中的四个项目提供组织形态学支持， 三维结构评估可行性项目。过程中 五年内，该中心将准备和分析600多只大鼠和小鼠， 标本和200个人体活检标本。将采用骨组织形态计量学 为了表征缺乏这些基因的转基因小鼠的骨骼表型， p53抑癌基因。骨组织形态计量学将被广泛应用 探讨甲状旁腺素的合成代谢作用机制及其相互作用 膳食钙缺乏和PT管理之间的关系。骨 将采用组织形态计量学来表征细胞和 甲状旁腺切除术对绝经后轻度甲状腺功能亢进妇女的结构影响 原发性甲状旁腺功能亢进骨组织形态计量学将用于探索 PTH在患者中的初始强烈合成代谢作用的机制 骨质疏松症将通过Micro-MRI评估结构变量， 与松质骨和 皮质骨结构这个核心将采用最先进的技术 并将带来超过15年的样本分析经验 和数据的解释。