GROWTH FACTORS DURING CELL TRANSDIFFERENTIATION IN NEWT OCULAR TISSUES

这项研究项目的长期目标是了解 细胞、形态和生化过程中发生的事件 有色虹膜上皮细胞(IECs)的转分化 成鱼眼虹膜背侧部绿色结节线虫进入晶状体 晶状体再生过程中的纤维。越来越多的人认识到，增长 因子作为有丝分裂原作用于各种类型的细胞，它们的 作用是通过细胞外基质相互作用和第二 信使已经引起了人们对涉及到 这些因素对再生系统的影响。将进行实验 无论是在体内还是体外。我们将重点分析 再生细胞的去分化和再分化阶段 进程。该项目将有以下具体目标：(1) 测定FGFs(成纤维细胞生长因子-1和成纤维细胞生长因子-2)、胰岛素样生长因子-1和转化生长因子-2的分布。 利用免疫细胞化学技术，在 IEC转分化；(2)检测成纤维细胞生长因子家族的作用 生长因子(成纤维细胞生长因子-1和成纤维细胞生长因子-2)、胰岛素样生长因子-1和转化生长因子-β对IEC生长的影响； 体外迁移、增殖和分化及其在细胞中的分布 IECs转分化过程中可能的相互作用，(3)到 成纤维细胞生长因子-1、成纤维细胞生长因子-2及其受体基因表达 IECs的转分化；以及，(4)确定生长因子 促进IECS差异化状态维护的要求 并在无血清培养条件下向晶状体细胞转分化。 在这个项目中，将努力获得关于 细胞脱分化过程的调控机制 将所获得的想法应用于去分化的期望 在癌变过程中发生的过程。这项研究项目将 继续为学生提供学习不同的机会 技术，让他们参与主要的科学活动，即 研究，并激励他们在不同的领域追求职业生涯 生物医学研究。

The long-term goal of this research project is to understand the cellular, morphological and biochemical events which occur during the transdifferentiation of pigmented iris epithelial cells (IECs) from the dorsal iris of the adult newt eye Notophthalmus viridescens into lens fibers during lens regeneration. The increasing knowledge that growth factors act as mitogens on a variety of cell types and that their effect is mediated by extracellular matrix interactions and second messengers has evoked interest in studies dealing with the effect of such factors on regenerating systems. Experiments will be conducted both in vivo and in vitro. We will emphasize analysis of the dedifferentiation and redifferentiation phases of the regenerative process. This project will have the following specific objectives: (1) to determine the distribution of FGFs (FGF-1 and FGF-2), IGF-1 and TGF- beta and their receptors, using immunocytochemical techniques, during IEC transdifferentiation; (2) to examine the effects of the FGF family (FGF-1 and FGF-2) of growth factors, IGF-1 and TGF-B on IEC outgrowth, migration, proliferation and differentiation in vitro, and their possible interactions during the transdifferentiation of IECs, (3) to study gene expression of FGF-1, FGF-2 and their receptors during the transdifferentiation of IECs; and, (4) to determine growth factor requirements that promote both maintenance of IECs differentiated state and their transdifferentiation into lens cells in serum-free medium. In this project efforts will be made to obtain basic information about the control mechanisms of the process of cell dedifferentiation with the expectation of applying the ideas obtained to the dedifferentiative process which occurs during carcinogenesis. This research project will continue to provide students with the opportunity to learn different techniques, to involve them in the main scientific activity that is research, and to motivate them to pursue careers in different areas of biomedical research.