GROWTH FACTORS DURING CELL TRANSDIFFERENTIATION IN NEWT OCULAR TISSUES

蝾螈眼组织细胞转移过程中的生长因子

• 批准号: 6219059
• 负责人: JOSE F FERNANDEZ
• 金额: $ 0.94万
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6219059
• 关键词: Urodela; alternatives to animals in research; cell differentiation; epidermal growth factor; eye regeneration; gene expression; growth factor; histogenesis; immunocytochemistry; in situ hybridization; insulinlike growth factor; iris; lens; northern blottings; organ culture; radionuclides; retinal pigment epithelium; transforming growth factors; western blottings

The long-term goal of this research project is to understand the cellular, morphological and biochemical events which occur during the transdifferentiation of pigmented iris epithelial cells (IECs) from the dorsal iris of the adult newt eye Notophthalmus viridescens into lens fibers during lens regeneration. The increasing knowledge that growth factors act as mitogens on a variety of cell types and that their effect is mediated by extracellular matrix interactions and second messengers has evoked interest in studies dealing with the effect of such factors on regenerating systems. Experiments will be conducted both in vivo and in vitro. We will emphasize analysis of the dedifferentiation and redifferentiation phases of the regenerative process. This project will have the following specific objectives: (1) to determine the distribution of FGFs (FGF-1 and FGF-2), IGF-1 and TGF- beta and their receptors, using immunocytochemical techniques, during IEC transdifferentiation; (2) to examine the effects of the FGF family (FGF-1 and FGF-2) of growth factors, IGF-1 and TGF-B on IEC outgrowth, migration, proliferation and differentiation in vitro, and their possible interactions during the transdifferentiation of IECs, (3) to study gene expression of FGF-1, FGF-2 and their receptors during the transdifferentiation of IECs; and, (4) to determine growth factor requirements that promote both maintenance of IECs differentiated state and their transdifferentiation into lens cells in serum-free medium. In this project efforts will be made to obtain basic information about the control mechanisms of the process of cell dedifferentiation with the expectation of applying the ideas obtained to the dedifferentiative process which occurs during carcinogenesis. This research project will continue to provide students with the opportunity to learn different techniques, to involve them in the main scientific activity that is research, and to motivate them to pursue careers in different areas of biomedical research.

本研究项目的长期目标是了解 细胞，形态和生化事件发生在 色素虹膜上皮细胞（IEC）的转分化 成年蝾螈眼的背侧虹膜绿背螈进入透镜 纤维在透镜再生。越来越多的人认识到， 这些因子在多种细胞类型上起有丝分裂原的作用， 作用是由细胞外基质相互作用介导的， 信使引起了人们对研究 这些因素对再生系统。实验将在 无论是在体内还是体外。我们将着重分析 再生细胞的脱分化和再分化阶段 过程 该项目将有以下具体目标：（1） 以确定FGF（FGF-1和FGF-2）、IGF-1和TGF-β 1在大鼠脑内的分布。 β及其受体，使用免疫细胞化学技术， IEC转分化;（2）检查FGF家族的作用 （FGF-1和FGF-2）的生长因子，IGF-1和TGF-β对IEC生长， 体外迁移、增殖和分化， IEC转分化过程中可能的相互作用，（3） 研究FGF-1、FGF-2及其受体在细胞凋亡过程中的基因表达， IEC的转分化;以及（4）确定生长因子 促进维持IEC差异化状态的要求 并在无血清培养基中转分化为透镜细胞。 在本项目中，将努力获得以下方面的基本信息： 细胞脱分化过程的调控机制 期望将所获得的思想应用于去微分 在致癌过程中发生的过程。该研究项目将 继续为学生提供学习不同 技术，让他们参与主要的科学活动， 研究，并激励他们在不同的领域追求事业， 生物医学研究