Core--Tissue Culture Core

组织培养核心将支持项目1、项目3和项目4中的组织培养活动。（项目2使用大鼠肝脏，项目5使用完整的转基因动物）。该核心将优化使用广泛的组织培养专业知识，积累在本单位在过去的18年。它将为本项目的研究人员获取和测试组织培养所需材料的质量提供一个中心设施。这些材料将包括胎牛血清（FBS），对其进行一致性测试，然后订购足够数量的胎牛血清，以便单批胎牛血清可以支持该计划项目的活动至少一年。新的组织培养塑料器皿在被广泛应用于项目实验室之前，也将由核心进行问题测试。核心将提供起始培养物，并在需要时准备特定实验的培养物。每进行一次培养，调查人员都要收费；该费用旨在鼓励从核心组织培养物的最佳使用。细胞将在核心中通过精细的方法使用标准化的方案生长。成纤维细胞的内部方案现已公布（附录）。这些程序通过培养生物年龄（CPDL）提供了成纤维细胞培养的高度标准化，以及对组织培养的其他方面（如支原体检测）的细致控制。肿瘤细胞，包括PC12细胞，将通过我们在这个实验室已经标准化的技术来培养。必要时，该核心也将制备初级神经元富集或胶质富集的培养物。核心的经验将有助于在任何项目中使用其他单元类型。组织培养核心的目的是增加组织培养设施，在实验室的具体调查员。它将为个体研究者的组织培养需求提供最佳支持，同时使每个研究者完全控制其调查所需的培养。

The Tissue Culture Core will support tissue culture activities in Project 1, Project 3, and Project 4. (Project 2 will use rat liver, and Project 5 will use intact transgenic animals). This Core will optimize the use of the extensive tissue culture expertise that has accumulated in this unit over the last 18 years. It will provide a central facility for obtaining and testing the quality of materials required for tissue culture by the investigators in this Program Project. The materials will include Fetal Bovine Serum (FBS), which is tested for consistency and then ordered in large enough amounts so that a single lot of FBS will support the activities of the Program Project for at least one year. New tissue culture plasticware will also be tested for problems by the Core before being used more widely within the Program Project laboratories. The Core will provide started cultures, and when requested, will prepare cultures for specific experiments. There will be a charge to the investigators for each culture; this fee is designed to encourage optimal use of tissue cultures from the core. Cells will be grown in the core by meticulous methods using well- standardized protocols. Inside the protocol for the fibroblasts has now been published (APPENDIX). These procedures provide a high degree of standardization of fibroblast cultures by biological age in culture (CPDL), as well as meticulous control of other aspects of tissue culture such as mycoplasma testing. Tumor cells, including PC12 cells, will be grown by techniques that we have already standardized in this laboratory. Where necessary, this core will also prepare primary neuron-enriched or glial- enriched cultures. The experience of the core will facilitate the use of other cell types for any of the PROJECTS. The Tissue Culture Core is designed to augment tissue culture facilities in the laboratories of the specific investigators. it will provide optimal support for the tissue culture needs of the individual investigators, while leaving each investigator in full control of the cultures needed for their investigation.