SECOND GENERATION TOOLS FOR FUNCTIONAL GENOMICS

申请者？S描述：易拉根将开发一种新的功能基因组学软件 帮助科学家使用基因组序列数据来解决生物学和 第二阶段的生物医学问题。该产品将需要 科学家超越了使用BLAST进行序列标注的经典方法 搜索以确定同源物，并假设两个蛋白质通过共同的 祖先必须有类似的功能。第一阶段表明， 这种数据库注释方法经常会产生误导，并且经常会遗漏 后生动物蛋白质中有意义的解释。第二阶段将 完善和全面开发第一阶段启动的软件。该软件将 用于识别在分支过程中功能可能发生更改的位置 Sequence系列，以及使用以下方法进行功能招募的位置 DNA序列的变化率。此外，要利用的工具 蛋白质/蛋白质和DNA/蛋白质的概率祖先序列 路线将得到充分发展。这些工具将检测遥远的同源基因， 识别序列数据库中的错误，并增强当前的方法以 基因发现。 建议的商业应用： 不可用

APPLICANT?S DESCRIPTION: EraGen will develop a new functional genomics software suite that helps scientists use genomic sequence data to solve biological and biomedical problems in this Phase II period. The product will take the scientist beyond classical approaches to sequence annotation, which use BLAST searches to identify homologs, and assume that two proteins related by common ancestry must have analogous functions. The Phase I period demonstrated that this approach to database annotation is often misleading, and frequently misses meaningful interpretations in metazoan proteins. The Phase II period will refine and fully develop the software initiated in Phase I. This software will serve to identify where function might have changed during divergence of a sequence family, and also where recruitment of function has occurred using rates of change from the DNA sequence. In addition, the tools to exploit probabilistic ancestral sequences for both protein/protein and DNA/protein alignments will be fully developed. These tools will detect distant homologs, identify errors across sequence databases, and augment current approaches to gene finding. PROPOSED COMMERCIAL APPLICATIONS: Not Available