Core--Demonstration

牙周病领域的研究确定了炎症过程中涉及的关键因素，包括；1)中性粒细胞的作用；2)脂质介质的作用；3)识别放大炎症的细菌过程；宿主先天免疫和体液免疫系统的作用。支持项目1-4，优化、集中和促进天然脂质介质的新颖、局部活性抗炎和抗pmn类似物的发现；该核心将提供并应用已建立的体内动物、细胞和器官模型，并进行体外分析，以：a)协助发现项目1-4中产生的新型、局部发生的抗pmn和抗炎化合物的结构/功能关系，并确定其在野生型和转基因兔子体内的作用。b)提供项目1-4细胞的流式细胞术分析。c)提供PMN体外和体内功能测定。D)提供和发展动物和细胞模型/试验，以评估牙周炎的新疗法。为该核心提供这些常规任务的集中位置将消除在其他项目中这些分析/模型的昂贵重复。该核心将优化生产交互和资源利用，并为其他项目提供有效和及时的访问。优先级为其他项目提供了有效和及时的新细胞和体内试验。试验/模型的优先排序将通过D核心项目首席研究员与项目项目首席研究员之间的单独会议以及项目项目团队的月度会议来完成。这些模型和检测的集中将促进这些新疗法在牙周病中的示范和有效性。

Studies in the area of periodontal disease have defined the crucial factors involved in the inflammatory process including; 1) the role of neutrophils; 2) the role of lipid mediators; 3) identification of bacterial processes in amplifying the inflammatory condition; and 4) the role of the hosts innate and humoral immune systems. In support of projects 1-4, and to optimize, centralize and facilitate the discovery of novel, topically active anti- inflammatory and anti-PMN analogues of naturally occurring lipid mediators; this Core will provide and apply established in vivo animal, cellular and organ models, as well as carry out in vitro assays to: a) assist in the discovery of structure/function relationships of novel, topically occurring anti-PMN and anti-inflammatory compounds generated in Projects 1-4 and establish their actions in vivo in wild type and genetically altered rabbits. b) provide flow cytometric analysis of cells from Project 1-4. c) provide PMN functional assays in vitro and in vivo. d) provide and develop animal and cellular models/assays for the evaluation of novel therapeutics in periodontitis. Providing a centralized location of these routine tasks to this Core will eliminate costly duplication of these assays/models within the other projects. This Core will optimize productive interactions and resource utilization and provide the other projects with efficient and timely access to novel. Prioritization of the provide the other projects with efficient and timely access to novel cell and in vivo assays. Prioritization of the assays/models will be accomplished through individual meetings between Core D's principal investigators and the Program Project principal investigators and during monthly meetings of the Program Project team. Centralization of these models and assays will facilitate the demonstration and the effectiveness of these novel therapeutics in periodontal disease.