Core--Demonstration

牙周病领域的研究已经明确了参与炎症过程的关键因素，包括：1)中性粒细胞的作用；2)脂质介质的作用；3)细菌过程在放大炎症状态中的识别；以及4)宿主天然免疫系统和体液免疫系统的作用。为支持项目1-4，优化、集中和促进发现新型、局部活性的抗炎和抗中性粒细胞天然脂质介体类似物；该核心将提供和应用已建立的体内动物、细胞和器官模型，并进行体外分析，以帮助发现项目1-4中产生的新型局部抗中性粒细胞和抗炎化合物的结构/功能关系，并确定它们在野生型和转基因兔体内的作用。B)提供项目1-4中的细胞的流式细胞仪分析。C)提供体外和体内的PMN功能检测。D)为评估牙周炎的新疗法提供和发展动物和细胞模型/分析。将这些例行任务集中放置到此核心将消除在其他项目中重复这些分析/模型的成本。这一核心将优化生产性互动和资源利用，并为其他项目提供高效和及时的新技术。为其他项目提供高效和及时的新细胞和体内分析的优先顺序。分析/模型的优先顺序将通过Core D的主要调查人员和计划项目主要调查人员之间的单独会议以及计划项目团队的每月会议来完成。这些模型和检测的集中化将促进这些新疗法在牙周病中的示范和有效性。

Studies in the area of periodontal disease have defined the crucial factors involved in the inflammatory process including; 1) the role of neutrophils; 2) the role of lipid mediators; 3) identification of bacterial processes in amplifying the inflammatory condition; and 4) the role of the hosts innate and humoral immune systems. In support of projects 1-4, and to optimize, centralize and facilitate the discovery of novel, topically active anti- inflammatory and anti-PMN analogues of naturally occurring lipid mediators; this Core will provide and apply established in vivo animal, cellular and organ models, as well as carry out in vitro assays to: a) assist in the discovery of structure/function relationships of novel, topically occurring anti-PMN and anti-inflammatory compounds generated in Projects 1-4 and establish their actions in vivo in wild type and genetically altered rabbits. b) provide flow cytometric analysis of cells from Project 1-4. c) provide PMN functional assays in vitro and in vivo. d) provide and develop animal and cellular models/assays for the evaluation of novel therapeutics in periodontitis. Providing a centralized location of these routine tasks to this Core will eliminate costly duplication of these assays/models within the other projects. This Core will optimize productive interactions and resource utilization and provide the other projects with efficient and timely access to novel. Prioritization of the provide the other projects with efficient and timely access to novel cell and in vivo assays. Prioritization of the assays/models will be accomplished through individual meetings between Core D's principal investigators and the Program Project principal investigators and during monthly meetings of the Program Project team. Centralization of these models and assays will facilitate the demonstration and the effectiveness of these novel therapeutics in periodontal disease.