Epidemiology and Immunobiology of HSV Infection

HSV 感染的流行病学和免疫生物学

• 批准号: 6348927
• 负责人: CHARLES G PROBER
• 金额: $ 14.58万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2001-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6348927
• 关键词: African American; adolescence (12-20); antibody formation; clinical research; communicable disease transmission; cooperative study; cytokine; epidemiology; helper T lymphocyte; herpes simplex virus 1; herpes simplex virus 2; human subject; leukocyte activation /transformation; microorganism immunology; protein biosynthesis; serology /serodiagnosis; sex behavior; sex partner; sexually transmitted diseases

There are limited data regarding the prevalence of HSV-2 infections among US adolescents. In addition, the functional mechanism and molecular targets of the initial immune responses that provide the foundation for controlling infection and promoting pathogenesis is largely unknown. Examining infection in adolescents who are presumably more immunologically "naive" and more likely to be diagnosed with a primary infection, compared to older individuals provides an important for evaluating the interaction among factors related to initial acquisition of infection, transmission, or progression to more severe sequelae including recurrent ulcers. Thus, adolescents present a unique opportunity to examine those factors which have direct relevance to vaccine development and treatment. Determining the prevalence of HSV-1 infection among adolescents and the interrelationship, if any, between seroprevalence of HSV-1 and HSV-2 will provide further important information regarding optimal HSV vaccine strategies. Specifically our aims are: 1. To determine sociodemographic and behavioral risk factors for prevalent and incident HSV-1 and HSV-2 infection among sexually active African-American adolescents. 2. To determine the natural history of cellular mediated immune responses in relation to clinical manifestations of HSV among infected individuals. We will assess the CD4 T-cell recognition of glycoproteins common to HSV-1 and HSV-2 (gB and gD), the type-specific gG2 protein and HSV-infected cell antigen, along with the Th1 and Th2 cytokine responses to stimulation with these antigens. 3. To determine immunologic risk factors for transmission of HSV-2 between adolescents and their sex partners over the course of the study among couples who are discordant on their HSV-2 serologic status. Transmission patterns will be assessed using gG2-based type-specific serologic assay, and then correlated with clinical histories of HSV infection, antibodies to heterologous virus, and duration of relationships.

关于美国青少年中HSV-2感染的患病率的数据有限。另外，最初免疫反应的功能机制和分子靶标为控制感染和促进发病机理提供基础是未知的。与老年人相比，检查大概是免疫学上更“天真”并且更可能被诊断为原发性感染的青少年的感染，这对于评估与最初获得感染，传播或进展到更严重的后续后期的相关因素之间的相互作用很重要，包括复发性溃疡。因此，青少年提供了一个独特的机会，可以检查与疫苗开发和治疗直接相关的因素。确定青少年中HSV-1感染的流行率以及HSV-1和HSV-2之间的相互关系（如果有的话）将提供有关最佳HSV疫苗策略的进一步重要信息。具体来说，我们的目的是：1。确定性活跃的非裔美国青少年中普遍存在和事件HSV-1和HSV-2感染的社会人口统计学和行为风险因素。 2。确定与受感染个体中HSV的临床表现有关的细胞介导的免疫反应的自然史。我们将评估对HSV-1和HSV-2（GB和GD）共同的糖蛋白的CD4 T细胞识别，类型特异性的GG2蛋白和HSV感染的细胞抗原以及TH1和TH2细胞因子对刺激的TH1和TH2细胞因子对这些抗原的反应。 3。确定在研究过程中，在研究过程中，对HSV-2的血清学位状态不一致的夫妇，在研究过程中，HSV-2在青少年及其性伴侣之间传播的免疫风险因素。传输模式将使用基于GG2的特异性血清学测定法进行评估，然后与HSV感染，异源病毒抗体和关系持续时间的临床历史相关。