INNER HAIR CELL PHYSIOLOGY IN INTACT PERFUSED COCHLEAS

完整灌注耳蜗的内毛细胞生理学

• 批准号: 6379396
• 负责人: JONATHAN H SIEGEL
• 金额: $ 11.07万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379396
• 关键词: acoustic nerve; auditory stimulus; basolateral membrane; calcium channel; cell morphology; chemical models; chloride channels; cochlea; ear hair cell; gerbil /jird; model design /development; neural transmission; neurotransmitters; organ culture; potassium channel; resting potentials; voltage /patch clamp

DESCRIPTION: This project seeks to understand how the responses to acoustic stimuli by inner hair cells (IHC) of the mammalian hearing organ are influenced by ionic currents passing through specific ion channels in the hair cell's basolateral membrane. A biophysical model predicts that among the effects of voltage-activated ion channels are likely to be a contribution to the adaptation of the hair cell to continuous sinusoidal stimulation and a hair cell equivalent of two-tone suppression. Whole-cell patch-clamp recording techniques will be carried out in intact cochleas removed from Mongolian gerbils and maintained by perfusion. Hair cell viability will be assessed using fluorescent dyes that indicate intracellular pH, membrane potential and synaptic vesicle recycling. Patch clamp data will be integrated into the biophysical hair cell model. Changes in membrane capacitance resulting from fusion of synaptic vesicles will be measured to characterize transmitter release. In addition to examining the basic biophysics of hair cell function, this work also contributes to the understanding of hair cell ionic homeostasis and thereby has relevance to understanding of hearing loss resulting from metabolic or other trauma to hair cells.

描述：该项目旨在了解如何响应声学 哺乳动物听觉器官的内毛细胞（IHC）的刺激是 受通过特定离子通道的离子电流的影响， 毛细胞基底外侧膜。 生物物理模型预测， 电压激活离子通道的作用可能是一种 对毛细胞适应连续正弦曲线的贡献 刺激和相当于双音抑制的毛细胞。 全细胞 膜片钳记录技术将在完整的耳蜗中进行 从蒙古沙鼠中取出并通过灌注维持。 毛细胞 将使用荧光染料评估存活力， 细胞内pH、膜电位和突触囊泡再循环。 贴片 钳数据将被整合到生物物理毛细胞模型中。 变化 突触囊泡融合产生的膜电容 测量以表征发射器释放。 除了检查 毛细胞功能的基本生物物理学，这项工作也有助于 了解毛细胞离子稳态，从而与 了解因代谢或其他创伤导致的听力损失， 毛细胞