PROPERTIES OF OLFACTORY RECEPTORS--NEURAL REGENERATION

嗅觉受体的特性——神经再生

• 批准号: 6370179
• 负责人: RICHARD M COSTANZO
• 金额: $ 25.37万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6370179
• 关键词: aging; anosmia; behavior test; brain injury; chemoreceptors; genetically modified animals; laboratory mouse; nervous system regeneration; olfactions; olfactory lobe; olfactory nerve; synaptogenesis; synaptophysin

DESCRIPTION: (provided by applicant) Damage to the olfactory nerves is thought to result in anosmia (loss of olfactory function) by preventing signals from odor receptor cells from reaching the brain. At the present time little hope is offered to those with impaired olfactory function. This project will combine molecular techniques with a nerve regeneration model to investigate the capacity of olfactory neurons to regenerate, replace, and repair damaged neurons in the central nervous system. Genetic markers introduced into a strain of transgenic mice will be used to trace regenerating olfactory nerves as they grow back to their target sites in the olfactory bulb. Experiments will determine if the regenerating nerve fibers can restore sensory maps in olfactory brain regions. Age related changes in sensory maps will also be investigated. Immunocytochemical methods will be used to determine the time course and location of new synapse formation during recovery from nerve transection. Behavioral experiments will determine the amount of reconnection needed to restore odor mediated behavior and if increasing odor exposure (odor therapy) helps to improve the time course of recovery. Results from this study could have important clinical implications for rehabilitation and recovery of function following brain injury. Findings could provide the foundation for future treatment strategies to help improve or restore function in individuals with anosmia and other brain related injuries.

描述：（由申请人提供）认为嗅觉神经受损 导致嗅觉丧失（嗅觉功能丧失）， 气味受体细胞到达大脑。目前，希望不大。 提供给嗅觉功能受损的人。这个项目将联合收割机 分子技术与神经再生模型，以研究 嗅觉神经元再生、替换和修复受损的能力 中枢神经系统中的神经元。引入菌株的遗传标记 转基因小鼠将被用来追踪再生的嗅觉神经，因为它们 长回嗅球的目标位置实验将 确定再生的神经纤维是否可以恢复感觉地图， 嗅觉脑区与年龄相关的感觉地图的变化也将是 研究了免疫细胞化学方法将用于确定时间 神经恢复过程中新突触形成的过程和位置 横切行为实验将确定重新连接的数量 需要恢复气味介导的行为，并且如果增加气味暴露（气味 治疗）有助于改善恢复的时间进程。本研究结果 可能对康复和恢复有重要的临床意义， 脑损伤后的功能。研究结果可以为以下方面提供基础： 未来的治疗策略，以帮助改善或恢复功能的个人 嗅觉丧失和其他脑损伤