EFFICACY STUDIES OF CHEMOPREVENTIVE AGENTS IN ANIMAL MOD

化学预防剂在动物模型中的药效研究

• 批准号: 6346798
• 负责人: BAHARA A REDDY
• 金额: $ 29.24万
• 依托单位: INSTITUTE FOR CANCER PREVENTION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6346798
• 关键词: cancer prevention; carcinogenesis; carcinogenesis inhibitor; chemoprevention; colon neoplasms; drug screening /evaluation; intestinal villi; laboratory rat; light microscopy; male; nonsteroidal antiinflammatory agent; small intestines

The objective of the study is to assess the efficacy of eight NO-releasing NSAIDs to reduce or inhibit colon carcinogenesis as measured by the aberrant crypt foci assay using male Fisher 344 rats treated in accordance with the two protocols described below. Measure aberrant crypt formation before and after the first carcinogen exposure, according to methods of Tudek, Bird, and Bruce. Eight week old rats are injected twice with15 mg of azoxymethane (AOM) per kg of body weight one week apart. The chemopreventive agent is administered beginning at 4 weeks after the first carcinogen dose and for another four weeks. the number of aberrant crypt foci per small intestine and for colon are determined. Also the number of aberrant crypts per foci from unsectioned methylene-blue stained tissue under low power light microscopy are being determined. The relative colon cancer chemoprevention efficacy using the three agents are being determined which were most effective in the crypt assay.

该研究的目的是评估八种释放 NO 的 NSAID 减少或抑制结肠癌发生的功效，这是通过使用按照下述两种方案治疗的雄性 Fisher 344 大鼠进行异常隐窝病灶测定来测量的。 根据 Tudek、Bird 和 Bruce 的方法，测量第一次接触致癌物之前和之后的异常隐窝形成。 八周龄大鼠注射两次每公斤体重 15 毫克氧化偶氮甲烷 (AOM)，间隔一周。 化学预防剂在第一次服用致癌剂后 4 周开始给药，并持续另外 4 周。 确定每个小肠和结肠的异常隐窝灶的数量。还正在确定低功率光学显微镜下来自未切片亚甲蓝染色组织的每个病灶的异常隐窝的数量。 正在确定使用这三种药物的相对结肠癌化学预防功效，哪一种在隐窝测定中最有效。

项目成果

NO-releasing NSAIDs and colon cancer chemoprevention: a promising novel approach (Review).

释放 NO 的 NSAID 和结肠癌化学预防：一种有前途的新方法（综述）。

• 发表时间: 2002
• 期刊: International journal of oncology
• 影响因子: 5.2
• 作者: Rigas,Basil;Williams,JennieL
• 通讯作者: Williams,JennieL

NO-NSAIDs and cancer: promising novel agents.

NO-NSAID 和癌症：有前途的新药。

• DOI: 10.1016/s1590-8658(03)00049-5
• 发表时间: 2003
• 期刊: Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
• 作者: Rigas,B;Kalofonos,H;Lebovics,E;Vagenakis,AG
• 通讯作者: Vagenakis,AG