IN VITRO SCREENING OF POTENTIAL CHEMOPREVENTIVE AGENTS

潜在化学预防剂的体外筛选

• 批准号: 6209320
• 负责人: STEVEN W D'AMBROSIO
• 金额: --
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6209320
• 关键词: 13 cis retinoate; acetylcysteine; apoptosis; biomarker; cancer prevention; carcinogenesis inhibitor; catechols; cell cycle; cell line; chemoprevention; cytotoxicity; dehydroepiandrosterone; difluoromethylornithine; dosage; drug screening /evaluation; dyes; epithelium; glutathione; oltipraz; oral mucosa; oral pharyngeal neoplasm; retinoate; selenomethionine; tissue /cell culture; tocopherols

The culture of normal oral epithelial cells and immortalized oral epithelial cell lines has been published (for example see Xu et al., Methods in Cell Science 18:31-39, 1996 and Sacks, Cancer and Metastasis Review, 15:27-51, 1996). Such cultures or preferably dysplastic oral epithelial cells are being used to screen potential chemopreventive agents. Initial growth inhibition or other cytotoxicity assays with the test chemopreventive agent present are being used to select appropriate concentration ranges for use in the subsequent transformation-inhibition assays. The levels of induction of three endpoints associated with inhibiting, reversing, or retarding the cancer process in response to exposure to potential chemopreventive agents are being explored. Normal oral epithelial cells and immortalized oral epithelial cell lines (dysplastic oral epithelial cells) are being used to screen potential chemopreventive agents. The endpoints chosen are amenable to quantitation of response. At least three endpoints shall be measured for response to chemopreventive agents. The endpoints include: retardation of cell cycle, increased glutathione content, and induction of apoptosis, changes in ploidy, proliferative index (PCNA, BrdU), Ki-67, MiB-1, p53, KS-5, nuclear pleomorphism index, RAR-beta, TGF-beta, and micronuclei. Appropriate controls are being simultaneously tested. The ED50 (50% effective dose) are being determined by a method determined by a standard protocol.

正常口腔上皮细胞和永生化口腔上皮细胞的培养