PROSTAGLANDIN PRODUCTION BY PRIMATE PERIOVULATORY FOLLICLE

成功受精，当亲本基因组结合时完成 在激活的卵母细胞内，对于有效的 生殖 微管构型的研究 牛和猪的受精卵发现精子 在这些家庭的受精过程中， 物种 此外，我们已经证明，来自繁殖的精子 公牛在组织微管的能力上的变化是可以预见的， 精子掺入，以及精子中心体变化影响 受精的结果。 该项目的目标是 在分子水平上描述相对亲本 在受精过程中对牛受精卵的贡献=s中心体。 我们对这个项目有两个目标。 目标1. 是招募 母亲的分子到精子中心体至关重要的 完成受精？ 目标二。 分子基础是什么 结合雄性和雌性前核的能动性的 在这些实验中获得的信息将增加我们的 基本的分子机制知识， 母牛受精过程中原核并置和基因组结合， 这是所有动物繁殖过程中的关键一步 此外，委员会认为， 它为测试男性生殖功能的新方法带来了希望 潜在的繁殖种马 这项研究还可能揭示一种新的 测定影响生育力的化合物的部位， 申请生育评估、生殖管理，以及 生殖毒理学，以及帮助开发新的方法， 用于提高家养物种的繁殖效率。 资金 USDA 9702358出版物Sutovsky P，Simerly C，Hewitson L，Schatten G.核孔复合物和环状薄片的组装促进 哺乳动物受精卵母细胞的正常原核发育。 J Cell Sci 111：2841-2854，1998.

Successful fertilization, completed when parental genomes unite within an activated oocyte, is critically important for efficient reproduction. Investigations on the microtubule configurations in bovine and porcine zygotes have led to the discovery that the sperm contributes the centrosome during fertilization in these domestic species. In addition, we have demonstrated that sperm from breeding bulls vary predictably in the ability to organize microtubules after sperm incorporation, and that variations in sperm centrosomes affect the outcome of fertilization. The goals of this project are to characterize, at the molecular level, the relative parental contributions to the bovine zygote=s centrosome during fertilization. We have two aims for this project. Aim 1. Is the recruitment of maternal molecules to the sperm centrosome essential for the completion of fertilization? Aim 2. What is the molecular basis of the motility which unites the male and female pronu clei ? The information obtained during these experiments will add to our fundamental knowledge about the molecular mechanisms leading to pronuclear apposition and genomic union during fertilization in cows, a key step in the reproductive process of all animals. Furthermore, it holds promise for novel assays to test male reproductive function in potential breeding studs. This research may also uncover a new site at which to assay for compounds affecting fertility, with applications for fertility assessment, reproductive management, and reproductive toxicology, as well as aid in developing new approaches for improving reproductive efficiency in domestic species. FUNDING USDA 9702358 PUBLICATIONS Sutovsky P, Simerly C, Hewitson L, Schatten G. Assembly of nuclear pore complexes and annulate lamellae promotes normal pronuclear development in fertilized mammalian oocytes. J Cell Sci 111:2841-2854, 1998.