STRUCTURE & FUNCTION ANALYSIS OF TOLEROGENIC PEPTIDES

我们与同事们合作，在 斯坦福大学医学院研究源自 表现出有趣的免疫调节特性的MHC分子。 这项工作的长期目标是详细了解 这些多肽诱导T细胞耐受的基础，以及 开发用于人类临床的潜在候选药物 免疫抑制疗法。我们的角色是执行一项 MHC衍生多肽的结构/功能分析 确定必要和充分的结构构件 用于免疫抑制活性。到目前为止，我们已经通过一个 一个碱基序列十二肽的“丝氨酸残基” 它们对人类的活动至关重要。这些衍生品中的一个和两个 其他类似物具有比亲本序列更好的活性。 我们计划继续这些研究，并将标记的多肽用作 用于受体纯化和生物物理的亲和配体 人物刻画。FAB-MS是确认 我们合成的多肽及其衍生物的结构 在这件事上

We have undertaken a project in collaboration with colleagues in Stanford University School of Medicine to study peptides derived from MHC molecules which exhibit interesting immunomodulatory properties. The long-term goals of this effort are to understand in detail the basis for induction of T cell tolerization by these peptides, and also to develop potential candidates for use in human clinical immunsuppressive therapy. Our role is to carry out a structure/function analysis of MHC-derived peptides in order to determine the structural elements that are necessary and sufficient for immunosuppressive activity. To date, we have found, through a "serine scane" amino acid residues of a base sequence dodecapeptide which are critical for activity. One of these derivatives, and two other analogs have activity superior to that of the parent sequence. We plan to continue these studies, and to use tagged peptides as affinity ligands for receptor purification and biophysical characterization. FAB-MS is n invaluable tool for confirming the structure of the peptides and peptide derivatives that we synthesize in this