STRUCTURE & FUNCTION ANALYSIS OF TOLEROGENIC PEPTIDES

我们与同事合作， 斯坦福大学医学院研究肽来源于 显示出令人感兴趣的免疫调节特性的MHC分子。 这项工作的长期目标是详细了解 这些肽诱导T细胞耐受的基础，以及 开发用于人类临床的潜在候选物 免疫抑制疗法 我们的任务是执行 MHC衍生肽的结构/功能分析， 确定必要和充分的结构要素 免疫抑制活性。 到目前为止，我们已经发现，通过一个 “丝氨酸扫描”氨基酸残基的碱基序列十二肽 这对活动至关重要。 其中一个导数， 其它类似物的活性上级于亲本序列。 我们计划继续这些研究，并使用标记的肽作为 用于受体纯化和生物物理的亲和配体 特征化 FAB-MS是一种非常宝贵的工具， 我们合成的肽和肽衍生物的结构 在这

We have undertaken a project in collaboration with colleagues in Stanford University School of Medicine to study peptides derived from MHC molecules which exhibit interesting immunomodulatory properties. The long-term goals of this effort are to understand in detail the basis for induction of T cell tolerization by these peptides, and also to develop potential candidates for use in human clinical immunsuppressive therapy. Our role is to carry out a structure/function analysis of MHC-derived peptides in order to determine the structural elements that are necessary and sufficient for immunosuppressive activity. To date, we have found, through a "serine scane" amino acid residues of a base sequence dodecapeptide which are critical for activity. One of these derivatives, and two other analogs have activity superior to that of the parent sequence. We plan to continue these studies, and to use tagged peptides as affinity ligands for receptor purification and biophysical characterization. FAB-MS is n invaluable tool for confirming the structure of the peptides and peptide derivatives that we synthesize in this