MASS SPECTRAL STUDIES OF PORPHYRINS & CHLOROPHYLLS

卟啉的质谱研究

• 批准号: 6308901
• 负责人: Kevin M Smith
• 金额: $ 0.99万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308901
• 关键词: bacteria; biological products; biomaterials; biomedical resource; health care; neoplasm /cancer; plants; proteins; radiology; spectrometry; structural biology

Porphyrins, chlorophylls, and bacteriochlorophylls are complex aromatic macrocycles which are of great biological significance, and also have novel and theoretically interesting mass spectra. In the situation where only small amounts of natural tetrapyrroles are available, mass spectrometry is virtually the only technique which can be used to obtain structural data on our materials. Primary information from mass spectrometry affords information on the various substituents on the macro-ring because the ring does not normally fragment. Mass spectra also help to establish the structures of unknown (synthetic or biological) porphyrins and chlorophylls be they unexpected reaction products or previously unknown natural substances. Our group in UC Davis focusses its efforts on synthetic and biosynthetic studies of porphyrins and chlorin systems which are the prosthetic groups in hemoglobins, myoglobins, cytochromes, catalases, peroxidases, chlorophylls, and bacteriochlorophylls. We are also heavily involved in the characterization and use of naturally derived photosensitizers for use in photodynamic therapy of malignant tumors. Mass spectrometry in these tetrapyrrole systems has beenshown, over the years, to be a vitally important component of the spectroscopic arsenal for structural investigation and chemical reactivity.

卟啉、叶绿素和细菌叶绿素很复杂 具有重要生物学意义的芳香大环化合物，以及 还具有新颖且理论上有趣的质谱。 在 仅存在少量天然四吡咯的情况 质谱分析实际上是唯一能够 用于获取我们材料的结构数据。 基本的 来自质谱的信息提供了各种信息 大环上的取代基，因为环通常不 分段。 质谱也有助于确定结构 未知的（合成的或生物的）卟啉和叶绿素 意想不到的反应产物或以前未知的天然物质。 我们在加州大学戴维斯分校的团队专注于合成和 卟啉和二氢卟酚系统的生物合成研究 血红蛋白、肌红蛋白、细胞色素、过氧化氢酶中的辅基， 过氧化物酶、叶绿素和细菌叶绿素。 我们也是 大量参与自然衍生的表征和使用 用于恶性肿瘤光动力治疗的光敏剂。 这些四吡咯系统中的质谱分析已表明， 多年来，成为光谱学的一个极其重要的组成部分 用于结构研究和化学反应的武库。