CORE--MOLECULAR DIAGNOSTICS FACILITY

核心——分子诊断设施

• 批准号: 6318300
• 负责人: JAMES R DOWNING
• 金额: $ 19.77万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6318300
• 关键词: biomedical facility; human genetic material tag; human tissue; molecular oncology; neoplasm /cancer diagnosis; neoplasm /cancer genetics; northern blottings; pediatric neoplasm /cancer; polymerase chain reaction

The purpose of this Core is to provide a centralized facility to rapidly evaluate the potential role of identified genes in pediatric cancer, using primary clinical samples and established pediatric cancer cell lines. The Core laboratory will investigate the level of expression of candidate genes by either semiquantitative RT-PCR or Northern blot analysis using probes and sequences provided by the primary investigators. In addition, detailed evaluations of the presence of mutations in candidate genes will be performed. These studies will involve evaluations for rearrangements, deletions, and point mutations using standard methodologies. The centralization of these activities will allow for an efficient utilization of available clinical material and will ensure accurate results free from potential contaminations from cloned sequences in the primary investigator's laboratory. In addition, the availability of detailed clinical information on the primary patients will allow for the rapid assessment of the clinical relevance of interesting findings. This Core will also aid in the evaluation and characterization of neoplastic growths identified in the murine transgenic and knockout/knockin strains generated.

该核心的目的是提供一个集中的设施， 评估已鉴定基因在儿科癌症中的潜在作用， 使用原始临床样品和已建立的儿科癌细胞 线 核心实验室将研究 通过半定量RT-PCR或北方印迹检测候选基因 使用由主要供应商提供的探针和序列进行分析 investigators. 此外，还详细评估了 将进行候选基因的突变。 这些研究将 包括重排、缺失和点突变的评估 使用标准方法。 这些活动的集中化 将允许有效利用可用的临床材料 并将确保准确的结果不受潜在的污染， 在主要研究者的实验室克隆序列。 此外，本发明还提供了一种方法， 关于主要患者的详细临床信息的可用性 将允许快速评估的临床相关性 有趣的发现 该核心还将有助于评估， 在小鼠中鉴定的肿瘤生长的表征 产生转基因和敲除/敲入菌株。