GENETIC REGULATION OF ACETYLCHOLINE SYNTHESIS

描述：拟议研究的长期目标是确定 土壤中胆碱能神经元功能的遗传和分子基础 线虫线虫 本研究的重点是 胆碱能基因座含有2个基因：cha-1为结构基因 胆碱乙酰转移酶（ChAT），合成胆碱的酶。 神经递质乙酰胆碱和unc-17是编码乙酰胆碱的基因。 突触囊泡乙酰胆碱转运体。 该研究有6个主要 目的： 1)目的：研究大鼠脑内胆碱能神经系统的发育， 胚胎和胚后发育通过监测外观 胆碱能标记物。 2，3）为了确定参与的反式作用因子， 胆碱能表型的控制和表达。 4、5）研究 特定类别的胆碱能神经元对整体神经元的贡献 神经系统的功能和生物体的行为。 6)到 研究神经元递质在发育过程中的作用。 neuron. 这项拟议中的研究将提供一个分子和遗传分析， 神经元成为胆碱能神经元，并应阐明的过程， 神经元分化并获得共同的神经递质 身份

DESCRIPTION: The long-term goal of the proposed research is to determine the genetic and molecular bases of cholinergic neuronal function in the soil nematode Caenorhabditis elegans. The focus of the present research is on the cholinergic locus, which contains 2 genes: cha-1 is the structural gene for choline acetyltransferase (ChAT), the enzyme which synthesizes the neurotransmitter acetylcholine, and unc-17 is the gene which encodes the synaptic vesicle acetylcholine transporter. The research has 6 primary aims: 1) To study the development of the cholinergic nervous system during embryonic and postembryonic development by monitoring the appearance of cholinergic markers. 2, 3) To identify trans-acting factors involved in the control and expression of the cholinergic phenotype. 4, 5) To study the contribution of specific classes of cholinergic neurons to the overall function of the nervous system and behavior of the organism. 6) To investigate the role of a neuron's transmitter on the development of the neuron. The proposed research will provide a molecular and genetic analysis of how neurons become cholinergic neurons and should elucidate the processes by which neurons become differentiated and achieve a common neurotransmitter identity.