BIOSYNTHESIS OF CELL WALL BETA 1,6 GLUCAN IN YEAST

SP捆绑袋的三维结构 SK受体是根据它们的跨膜来定义的 通过确定α-螺旋和胞外区的位置 一系列SP和SK衍生物的共价键合 光反应性氨基酸对苯甲酰苯丙氨酸的间距为 两者的氨基酸序列位置不同 多肽。对于每个光亲和配体，其氨基酸残基 正在确定成为共价标记的结合位点 通过对最初位于 利用位点特异性抗体和质量进行初级测序 光谱分析。相似性与区别性的对比分析 这些绑定域的功能应该可以让我们深入了解 多肽选择性和受体激活的分子基础。一个 相关项目是确定附着物的地点 非肽SP的氚化光反应叠氮衍生物 拮抗剂CP-96,345。对两国关系的认识 多肽激动剂和非肽拮抗剂的部位可以 提供对此拮抗作用的机制的洞察 复合体，以及从整个项目中获得的信息 可促进临床治疗药物的合理设计 这些多肽参与的实体。

The three-dimensional structure of the binding pockets of the SP and SK receptors is being defined with respect to their transmembrane alpha-helices and extracellular domains by determining the sites of covalent attachment of a series of SP and SK derivatives containing the photoreactive amino acid p-benzoylphenylalanine spaced at different positions along the amino acid sequences of the two peptides. For each photoaffinity ligand, the amino acid residues of the binding site that becomes covalently labelled are being determined by microsequencing of purified receptor fragments initially located in the primary sequence with the aid of site-specific antibodies andmass spectrometry. A comparative analysis of the similar and distinctive features of these binding domains should provide insight into the molecular basis of peptide selectivity and receptor activation. A related project is determination of the site of attachment of a tritiated photoreactive azide derivative of the nonpeptide SP antagonist CP-96,345. An understanding of the relationship between the sites for peptide agonists and the nonpeptide antagonist may provide insight into the mechanism of the antagonist effects of this compound, and the information to be gained from the project as a whole may advance the rational design of therapeutic agents for clinical entities in which these peptides participate.