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GENES CAUSING SPONTANEOUS OBESITY

GENES CAUSING SPONTANEOUS OBESITY
导致自发性肥胖的基因
批准号:
6381143
负责人:
CRAIG H WARDEN
金额:
$24.86万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-06-01 至 2003-05-31

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中文摘要
翻译
本研究的长期目标是探讨的机制
英文摘要
The long-term goal of this research is to investigate the mechanisms of spontaneous obesity in mice. Previous work has identified 4 chromosomal regions (mouse chr. 6,7,12,and 15) or loci (QTLs) that contribute to obesity in spontaneously obese BSB mice. BSB mice were produced by a backcross of (Mus spretus x C57BL/6J) F1 x B6. Preliminary data on an available congenic mouse strains confirm that the locus on chr. 7 affects adiposity. The objective of this present proposal is to identify the genes underlying these obesity loci. Congenic mouse strains carrying the spretus chromosomal regions as donor DNA on the B6 background will be created. As co-incident QTLs for obesity, plasma cholesterol and hepatic lipase (HL) activity on mouse chr. 7 were found, the already constructed B6 HL knockout will used to test the hypothesis that alterations of HL activity determine these cholesterol and/obesity loci. BSB backcrosses with the HL KO B6 mouse will yield animals both homozygous and heterozygous for the HL knock-out. QTLs at chromosome 7; will be compared and contrasted in these two groups. The mouse chr. 6,7, and 1 loci include within their 90 percent confidence intervals, respectively, the obese, tubby, and uncoupling protein 2-genes. Molecular and biochemical studies of these candidate genes will be performed to test whether differences are likely to explain the observed effect on the trait. If there are differences in the coding portion of the spretus and B6 leptin, then the biological consequence of the differences will be tested. The chromosome 6 locus was linked to just one of the four fat pads measured in BSB mice. Leptin mRNA levels in the four fat pads will be determined to examine their correlations with plasma leptin and fat pad sizes. They have already found that spretus and B6 UCP2 differ for 2 amino acids, so uncoupling activity of UCP2 from these strains will be examined.
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Positional cloning of obesity genes from congenic mice
Positional cloning of obesity genes from congenic mice
Positional cloning of obesity genes from congenic mice
Positional cloning of obesity genes from congenic mice
国内基金
海外基金
PDLIM3-Cholesterol-SMO轴调控SHH通路激活及其在髓母细胞瘤中的功能研究
  • 批准号:
    82072798
  • 项目类别:
    面上项目
  • 资助金额:
    55.0万元
  • 批准年份:
    2020
  • 负责人:
    张丽
  • 依托单位:
以促内涵体逃逸聚合物PEG-P[Asp(TEP)]-cholesterol为载体构建双级脑靶向基因传递系统沉默BACE1基因的研究