CORE--GENE EXPRESSION AND MOLECULAR GENETICS

核心——基因表达与分子遗传学

• 批准号: 6358989
• 负责人: ELLEN L KITTLER
• 金额: $ 15.38万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-29 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6358989
• 关键词: biomedical facility; clone cells; gene expression; hematopoietic stem cells; molecular genetics; nucleic acid probes; polymerase chain reaction; transfection /expression vector

The Gene Expression & Molecular Genetics Core (B) is responsible for molecular biology support and cloning/expression studies as well as providing reagents to member projects. The types of services and supplies needed will, of course, vary between projects; however, the overall goal of the GEMG Core is to ensure that the members have the reagent, techniques and assistance needed to perform these studies. 1. Standardized reagents - enzymes, primers, PCR master mixes, probes. 2. Banking of plasmids, vectors, RNA from synchronized cells, etc. 3. In Situ PCR and RT=PCR 4. Establishing producer/packaging cell lines 5. Viral titer, supernatant harvest and storage 6. Validation assays - thymidine uptake, colony RT-PCR, colony PCR 7. Additional manpower for large procedures, e.g. transplants

基因表达和分子遗传学核心(B)负责 分子生物学支持和克隆/表达研究以及 为会员项目提供试剂。服务和用品的类型 当然，所需的内容因项目而异；然而， GEMG的核心是确保成员拥有试剂、技术 以及进行这些研究所需的协助。 1.标准化试剂--酶、引物、聚合酶链式反应标记物、探针。 2.来自同步化细胞的质粒库、载体库、RNA库等 3.原位聚合酶链式反应和RT=聚合酶链式反应 4.建立生产/包装细胞系 5.病毒滴度、上清液收获和储存 6.验证分析--胸腺嘧啶核苷摄取、菌落RT-PCR、菌落PCR 7.大型手术的额外人手，例如移植