Core--Molecular biology and cell culture

核心--分子生物学与细胞培养

• 批准号: 6313247
• 负责人: PHILIP A PATSTON
• 金额: $ 21.47万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-22 至 2004-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6313247
• 关键词: biomedical facility; cell line; molecular biology; plasmids; site directed mutagenesis; tissue /cell culture; transfection; transfection /expression vector

The Molecular Biology and Cell Culture Core will provide a centralized facility for carrying out all site-directed mutagenesis experiments, construction of new expression plasmids and creation of bacterial and baby hamster kidney cell lines expressing the desired proteins required by the P.I.s of the four projects. Construction of plasmids for e3xpression of new serpins will be done in consultation with the appropriate P.I. The core will occupy common space with the Protein Expression and Purification core. The Administrative Director will be responsible for overnight of the core and for ensuring equitable service to all of the projects.

分子生物学和细胞培养核心将提供一个集中的设施，用于进行所有定点突变实验，构建新的表达载体，并创建表达四个项目的P.I.S所需所需蛋白质的细菌和幼年仓鼠肾脏细胞系。构建新的蛇类e3表达的质粒将与适当的P.I.协商进行。核心将与蛋白质表达和纯化核心占据共同的空间。行政主任将负责为核心项目提供通宵服务，并确保向所有项目提供公平服务。