Core--Molecular biology and cell culture

分子生物学和细胞培养中心将提供一个集中的设施，用于进行所有定点诱变实验，构建新的表达质粒，并创建细菌和小仓鼠肾细胞系，表达四个项目P.I.s所需的所需蛋白质。构建用于表达新serpins的质粒将与适当的pi协商完成，核心将与蛋白质表达和纯化核心占用公共空间。行政主任将负责核心的夜间工作，并确保对所有项目提供公平的服务。

The Molecular Biology and Cell Culture Core will provide a centralized facility for carrying out all site-directed mutagenesis experiments, construction of new expression plasmids and creation of bacterial and baby hamster kidney cell lines expressing the desired proteins required by the P.I.s of the four projects. Construction of plasmids for e3xpression of new serpins will be done in consultation with the appropriate P.I. The core will occupy common space with the Protein Expression and Purification core. The Administrative Director will be responsible for overnight of the core and for ensuring equitable service to all of the projects.