REACTION OF B12 COMPOUNDS WITH NITRIC OXIDE AND NITROUS OXIDE

描述（改编自申请）：本研究的长期研究 了解维生素B12化合物在酶促反应中的作用， 反应并就其化学性质而言作为治疗剂 特性.该研究项目的一些具体目标是 研究氮氧化物N 0和N20与 维生素B12（钴胺素）化合物在体外，以了解其 对蛋氨酸合成酶B12-MS酶促反应的影响， 有效地隔离体内创伤诱导的NO。一个假设 是NO和N2 O与Co（II）和Co（I）形式的 钴胺素会影响酶的功能。具体研究包括 研究了NO、N_2O与 在三个氧化位点的柯啉类化合物，使用电化学 测量，紫外-可见光谱，共振拉曼光谱（RRS）， 表面增强共振（EPR）光谱。形成的氮物种 将通过GC-MS来研究作为反应产物的还原剂。 激活的裂解机制 还将研究甲基钴胺素和腺苷钴胺素。目的是 测量平衡和腺苷钴胺素也将进行研究。的 目的是测量平衡结合常数，氧化还原反应速率， 和钴胺素氧化还原电对的电化学还原电位， 以确定其亚硝酰复合物中NO结合的结构。 这些结果应该有助于理解NO和N20在 灭活MS-B12和钴胺素如何解毒生物体与升高 实验还将研究溶剂和pH值 络合物的平衡和动力学性质的影响。

Description (Adapted from Application): The long-term of this study are to understand the functions of vitamin B12 compounds in enzymatic reactions and as therapeutic agents in terms in terms of their chemical properties. Some specific goals of the research project are to investigate the interactions of nitrogen oxides, N0 and N20, with vitamin B12 (cobalamin) compounds in vitro in order to understand their effects on enzymatic reactions of methionine synthase B12-MS, and their effectiveness for sequestering trauma-induced NO in vivo. One hypothesis is that redox reactions of NO and N20 with the Co(II) and Co(I) forms of cobalamin can affect enzyme functioning. Specific studies include the complexes formed and the redox behavior of systems of NO, N20 with corrinoid compounds in three oxidation sites using electrochemical measurements, UV-Vis spectroscopy, resonance Raman spectroscopy (RRS), surface enhanced resonance (EPR) spectroscopy. Nitrogen species formed as reaction products will be investigated by GC-MS. The reductive cleavage mechanism for the activation for the activation of methylcobalamin and adenosylcobalamin will also be studied. The aims is to measure equilibrium and adenosylcobalamin will also be studied. The aim is to measure equilibrium binding constants, redox reaction rates, and electrochemical reduction potentials for cobalamin redox couples and to determine the structure of NO binding in their nitrosyl complexes. These results should help in understanding how NO and N20 work in deactivating MS-B12 and how cobalamine detoxify organisms with elevated levels of NO. Experiments will also investigate the solvent and pH effects of the equilibrium and kinetic properties of the complexes.