Toxokinetics of Swainsonine--Nutrient Metabolism Effects

目标和目的：该项目的长期目标包括：1)培训学生了解毒理学和营养代谢的原理；2)确定苦马豆素的吸收动力学和模式；3)确定苦马豆素对营养代谢的影响。为了实现这些目标，本项目将提出四个具体目标。具体目标#1是确定苦马豆素的吸收动力学。具体目标#2是确定苦马豆素对营养物质消化和流经消化道的影响。具体目的#3是确定苦马豆素的海绵状组织流量。具体目标#4是确定苦马豆素对所选营养素的海绵组织流量的影响。苦马豆素是一种天然植物。在临床上，苦马豆素已显示出作为抗肿瘤药物的前景。因此，苦马豆素已显示出作为抗肿瘤药物的前景。因此，苦马豆素正被积极探索作为癌症的治疗方法。虽然苦马豆素的化学治疗特性已经得到了广泛的研究，但关于苦马豆素的药代动力学特性已被广泛研究的数据很少或根本没有，如果我们要开发苦马豆素作为癌症治疗的口服给药方案和营养支持策略，关于苦马豆素的药代动力学、苦马豆素的作用或对营养代谢的影响的数据很少或根本没有。将对动物进行外科插管/插管，以便于收集消化和血液样本，以进行苦马豆素分析和营养代谢物分析。此外，还将收集相同的肠上皮细胞用于微囊转运研究。这些研究将提供一些有关苦马豆素的吸收及其对营养代谢的影响的第一批数据。

Goals and Aims: The long term goals of this project include: 1) training students in the principles of toxicology and nutrient metabolism, 2) defining the kinetics and mode of swainsonine absorption, and 3) defining the effects of swainsonine on nutrient metabolism. In order to accomplish these goals, four specific aims will be addressed in this project. Specific Aim #1 is to define the absorption kinetics of swainsonine Specific Aim #2 is to define the effects of swainsonine on nutrient digestion and flow through the digestive tract. Specific Aim #3 is to define the spanchnic tissue flux of swainsonine. Specific Aim #4 is to define the effects of swainsonine on spanchnic tissue flux of selected nutrients. Swainsonine is a naturally occurring plant. Clinically, swainsonine has shown promise as an anti-tumor agent. As such, swainsonine has shown promise as an anti-tumor agent. As such, swainsonine is actively being explored as a treatment for cancer. Although the chemotherapeutic properties of swainsonine have been extensively studied, little or no data exists concerning the pharmacokinetics properties of swainsonine have been extensively studied, little or no data exists concerning the pharmacokinetics of swainsonine or the effects of swainsonine or the effects on nutrient metabolism if we are to develop oral dosing protocols and nutritional support strategies for the use of swainsonine as a cancer treatment. Animals will be surgically cannulated/catheritized to facilitate the collection of digest and blood samples for swainsonine analysis and nutrient metabolite analysis. In addition, same intestinal epithelia will be collected for microvesicle transport studies. These studies will provide some of the first data concerning the absorption of swainsonine and its effects on nutrient metabolism.