CELL CYCLE BIOLOGY OF DOC-1

这项研究项目的主要目标是调查 推测抑癌基因缺失的分子机制 口腔癌-1(DOC-1)抑制恶性转化表型 口腔角质形成细胞。将提供的数据将支持这样的假设 DOC-1通过调节癌细胞的进程发挥作用 在细胞周期中以一种方式导致显著增加 在细胞的积累上处于S期。详细的研究将在 目的是阐明DOC-1在细胞周期中的作用。 此应用程序包含四个具体目标：(1)确定 Doc-1在人体组织中的表达模式；92)以确定其作用 磷酸化在DOC-1蛋白功能调节中的作用；(3) 研究DOC-1在细胞周期中的作用；以及(4)确定 DOC-1的职能合作伙伴。 拟议的研究将由一组积极的调查人员进行 在细胞周期和癌症研究领域，与 进行另外两个研究项目的研究人员以及 与细胞培养和组织库和病理核心将确保 这两项研究在临床层面得到了验证。

The major objective of this research project is to investigate the molecular mechanism whereby the putative tumor suppressor gene deleted-in oral cancer-1 (doc-1) suppresses transformation phenotypes of malignant oral keratinocytes. Data will be presented to support the hypothesis that doc-1 exerts its effect on cancer cells by regulating their progression through the cell cycle in a manner that results in a significant increase in the accumulation of cells in the S phase. Detailed studies will be conducted to elucidate the role of doc-1 in the cell cycle. This application encompasses four specific aims: (1) to determine the pattern of expression of doc-1 in human tissues; 92) to determine the role of phosphorylation in the regulation of Doc-1 protein function; (3) to examine the role of doc-1 in the cell cycle; and (4) to identify the functional partners of Doc-1. The proposed studies will be conducted by a team of investigators active in the field of cell cycle and cancer research Close collaborations with the investigators conducting the other two research projects as well as with the Cell Culture and Tissue Bank and Pathology Cores will ensure that these two studies are validated at the clinical level.