CELL CYCLE BIOLOGY OF DOC-1

DOC-1 的细胞周期生物学

• 批准号: 6300863
• 负责人: David T Wong
• 金额: $ 18.13万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300863
• 关键词: cell cycle; cell line; gene expression; human tissue; molecular oncology; mouth neoplasms; neoplastic transformation; phosphorylation; squamous cell carcinoma; tumor suppressor genes

The major objective of this research project is to investigate the molecular mechanism whereby the putative tumor suppressor gene deleted-in oral cancer-1 (doc-1) suppresses transformation phenotypes of malignant oral keratinocytes. Data will be presented to support the hypothesis that doc-1 exerts its effect on cancer cells by regulating their progression through the cell cycle in a manner that results in a significant increase in the accumulation of cells in the S phase. Detailed studies will be conducted to elucidate the role of doc-1 in the cell cycle. This application encompasses four specific aims: (1) to determine the pattern of expression of doc-1 in human tissues; 92) to determine the role of phosphorylation in the regulation of Doc-1 protein function; (3) to examine the role of doc-1 in the cell cycle; and (4) to identify the functional partners of Doc-1. The proposed studies will be conducted by a team of investigators active in the field of cell cycle and cancer research Close collaborations with the investigators conducting the other two research projects as well as with the Cell Culture and Tissue Bank and Pathology Cores will ensure that these two studies are validated at the clinical level.

本研究项目的主要目的是调查 肿瘤抑制基因缺失的分子机制 口腔癌-1（DOC-1）抑制恶性肿瘤的转化表型 口腔角质形成细胞将提供数据以支持以下假设： doc-1通过调节癌细胞的进展来发挥其作用 通过细胞周期的方式， 在S期细胞的积累。详细研究将在 以阐明DOC-1在细胞周期中的作用。 本申请包括四个具体目标：（1）确定 DOC-1在人体组织中的表达模式; 92）以确定其作用 磷酸化在调节Doc-1蛋白功能中的作用;（3） 检测doc-1在细胞周期中的作用;以及（4）鉴定 Doc-1的功能合作伙伴。 拟议的研究将由一组积极参与的研究人员进行。 在细胞周期和癌症研究领域与 进行另外两个研究项目的研究人员以及 与细胞培养和组织库和病理核心将确保， 这两项研究在临床层面得到验证。