TOC COMPLEX IN PROTEIN TARGETING TO CHLOROPLASTS

该提案中的实验联合收割机在体内的研究， Schnell博士将研究拟南芥突变体的影响， 叶绿体生物发生的TOC复合体，生物化学研究的目的是 阐明TOC复合物的各种亚基的功能作用， 膜转位本补助金提出了5个具体目标 应用程序.第一个目的涉及Toc 34、75和77的无效突变体的研究。 159在A和表达这些突变形式的后果 proteins.这项工作是与Kessler博士正在进行的合作的一部分， 苏黎世联邦理工学院的A. thaliana genetics.第二个目标与 第一个，因为它将使用类似的方法来探索功能， Toc 132和Toc 120，他们发现的两个基因似乎是 属于同一个蛋白质家族。第三个目标侧重于功能 第159章.具体而言，建议研究这一作用 GT3在识别从胞质溶胶输入的前蛋白质中起作用， 叶绿体有人建议寻找其他蛋白质， 这种识别是通过使用已建立的蛋白质-蛋白质相互作用的方法来实现的。 其他蛋白质是TOC复合物的一部分，目标4和5将在体外使用 研究它们在蛋白质输入中的潜在调节作用及其 联系地图

The experiments in this proposal combine in vivo studies in Arabidopsis thaliana, where Dr. Schnell will study the influence of mutants of the TOC complex of chloroplast biogenesis, with biochemical studies aimed to elucidate the functional role of various subunits of the TOC complex in membrane translocation. There are 5 specific aims proposed in this grant application. The first aim involves the study of null mutants of Toc34,75 and 159 in A. thaliana and consequences of expressing mutated forms of these proteins. This work is part of an ongoing collaboration with Dr. Kessler at ETH, Zurich, an expert in A. thaliana genetics. The second aim is related to the first one, in that it will use similar methods to explore the function of Toc 132 and Toc 120, two genes they have discovered that appear to be members of the same family of proteins. The third aim focuses on the functional dissection of Toc 159. Specifically it is proposed to study the role of this GTPase in the recognition of pre-proteins that are imported from the cytosol to the chloroplast. It is proposed to search for other proteins that facilitate this recognition by using established methods of protein-protein interaction. Other proteins are part of the TOC complex, and aims 4 and 5 will use in vitro assays to study their potential regulatory role in protein import and their contact map.