TOC COMPLEX IN PROTEIN TARGETING TO CHLOROPLASTS
TOC COMPLEX IN PROTEIN TARGETING TO CHLOROPLASTS
批准号:
6387250
负责人:
DANNY J. SCHNELL
金额:
$23.79万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-07-01 至 2004-06-30
中文摘要
该提案中的实验联合收割机在体内的研究,
Schnell博士将研究拟南芥突变体的影响,
叶绿体生物发生的TOC复合体,生物化学研究的目的是
阐明TOC复合物的各种亚基的功能作用,
膜转位本补助金提出了5个具体目标
应用程序.第一个目的涉及Toc 34、75和77的无效突变体的研究。
159在A和表达这些突变形式的后果
proteins.这项工作是与Kessler博士正在进行的合作的一部分,
苏黎世联邦理工学院的A. thaliana genetics.第二个目标与
第一个,因为它将使用类似的方法来探索功能,
Toc 132和Toc 120,他们发现的两个基因似乎是
属于同一个蛋白质家族。第三个目标侧重于功能
第159章.具体而言,建议研究这一作用
GT3在识别从胞质溶胶输入的前蛋白质中起作用,
叶绿体有人建议寻找其他蛋白质,
这种识别是通过使用已建立的蛋白质-蛋白质相互作用的方法来实现的。
其他蛋白质是TOC复合物的一部分,目标4和5将在体外使用
研究它们在蛋白质输入中的潜在调节作用及其
联系地图
英文摘要
The experiments in this proposal combine in vivo studies in
Arabidopsis thaliana, where Dr. Schnell will study the influence of mutants of
the TOC complex of chloroplast biogenesis, with biochemical studies aimed to
elucidate the functional role of various subunits of the TOC complex in
membrane translocation. There are 5 specific aims proposed in this grant
application. The first aim involves the study of null mutants of Toc34,75 and
159 in A. thaliana and consequences of expressing mutated forms of these
proteins. This work is part of an ongoing collaboration with Dr. Kessler at
ETH, Zurich, an expert in A. thaliana genetics. The second aim is related to
the first one, in that it will use similar methods to explore the function of
Toc 132 and Toc 120, two genes they have discovered that appear to be members
of the same family of proteins. The third aim focuses on the functional
dissection of Toc 159. Specifically it is proposed to study the role of this
GTPase in the recognition of pre-proteins that are imported from the cytosol to
the chloroplast. It is proposed to search for other proteins that facilitate
this recognition by using established methods of protein-protein interaction.
Other proteins are part of the TOC complex, and aims 4 and 5 will use in vitro
assays to study their potential regulatory role in protein import and their
contact map.
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会议论文
TOC COMPLEX IN PROTEIN TARGETING TO CHLOROPLASTS
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批准号:6191218
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项目类别:
-
资助金额:$0.0万
-
财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
Role of the TOC complex in plastid protein import
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批准号:6917638
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项目类别:
-
资助金额:$29.24万
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财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
TOC COMPLEX IN PROTEIN TARGETING TO CHLOROPLASTS
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批准号:6607132
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项目类别:
-
资助金额:$23.79万
-
财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
Role of the TOC complex in plastid protein import
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批准号:8685990
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项目类别:
-
资助金额:$8.36万
-
财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
Role of the TOC complex in plastid protein import
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批准号:8320161
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项目类别:
-
资助金额:$29.29万
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财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
Role of the TOC complex in plastid protein import
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批准号:8137596
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项目类别:
-
资助金额:$29.25万
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财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
Role of the TOC complex in plastid protein import
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批准号:7257024
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项目类别:
-
资助金额:$25.69万
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财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
Role of the TOC complex in plastid protein import
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批准号:8495352
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项目类别:
-
资助金额:$28.22万
-
财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
Role of the TOC complex in plastid protein import
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批准号:7077760
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项目类别:
-
资助金额:$26.48万
-
财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
TOC COMPLEX IN PROTEIN TARGETING TO CHLOROPLASTS
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批准号:6402030
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项目类别:
-
资助金额:$23.79万
-
财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
Role of the TOC complex in plastid protein import
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批准号:9272575
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项目类别:
-
资助金额:$23.05万
-
财政年份:2000
-
负责人:DANNY J. SCHNELL
-
依托单位:
TOC COMPLEX IN PROTEIN TARGETING TO CHLOROPLASTS
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批准号:6520325
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项目类别:
-
资助金额:$23.79万
-
财政年份:2000
-
负责人:DANNY J. SCHNELL
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依托单位:
海外基金