CORE--VIRUS AND CELL BIOLOGY

病毒和细胞生物学核心[VCBC]的目的是支持 拟议计划项目赠款[PPG]中的项目1-4。VCBC将 参与本PPG建议的以下调查：(1)。这个 从尿液中鉴定促进剂的作用机制 表现出抗KS活性的孕妇；与世隔绝和 孕妇尿液中各种因素的特征 抗KS活性；(3)Kaposi肉瘤与KS的关系 人类疱疹病毒HHV8是潜在的致病因素；以及 KS细胞系的分子决定因素。VCBC一直积极地 参与开发艾滋病的体外检测和细胞模型 有使用体外试验的经验，旨在研究各种 KS的几个方面。VCBC将提供集中服务和生物 PPG中项目1-4的样本，并确保一致的质量和 试剂和化验的一致性。VCBC目标，其中包括 分为六个具体目标，分别是：接收和监测库存水平 PPG中的所有生物材料，并保持所有生物材料 在最佳储存条件下的材料；提供试剂[病毒， 细胞、单抗和合成肽]对个体 调查人员并进行生物测试，以促进 在个别项目中提出的调查；将数据与 动物核心设施[核心B]，用于向项目1-4汇报。 维护材料，提供病毒库存和细胞，并执行 和/或通过VCBC协调生物检测将确保 该PPG所有项目的生物检测都是由 经过筛选的标准化试剂，从而便于在 所有项目生成的数据集。

The purpose of the Virus and Cell Biology Core [VCBC] is to support Projects 1-4 in the proposed Program Project Grant [PPG]. The VCBC will participate in the following investigations proposed in this PPG: (1). The identification of mechanisms of action of actors from the urine of pregnant women that exhibit anti-KS activity; (2). The isolation and characterization of factors from the urine of pregnant women that exhibit anti-KS activity; (3) The relationship between Kaposi's sarcoma (KS) and the human herpesvirus HHV8 as a potential etiologic factor; (4). And the molecular determinants of KS cell lines. The VCBC has been actively involved in developing in vitro assays and cellular models for AIDS and has experience working with in vitro assays designed to study various aspects of KS. The VCBC is to provide centralized services and biological samples for Projects 1-4 in the PPG and to assure consistent quality and uniformity of reagents and assays. The VCBC goals, which are incorporated into six Specific Aims, are: to receive and monitor the inventory levels of all biological material in the PPG and to maintain all biological materials at optimal storage conditions; to provide reagents [viruses, cells, monoclonal antibodies, and synthetic peptides] to individual investigators and to perform bioassays to further the Specific Aims of the investigations proposed in the individual projects; to collate data with the Animal Core Facility [Core B] for reporting back to Projects 1-4. Maintaining materials, providing virus stocks and cells, and performing and/or coordinating bioassays through the VCBC will ensure that the biological assays for all the projects of this PPG are conducted with screened, standardized reagents, thus facilitating comparisons among the data sets generated by all the projects.