Core--Xiphophorus Genetic Stock Center

核心--剑尾鱼种质中心

• 批准号: 6300561
• 负责人: STUART W CALHOUN
• 金额: $ 27.06万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-10 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300561
• 关键词: Osteichthyes; alternatives to animals in research; animal breeding; animal colony; animal genetic material tag; biomedical facility; genetic models; genetic registry /resource /referral center

Description: (Applicant's Description) The Xiphophorus Genetic Stock Center at Southwest Texas State University will serve as the core facility for the Program Project because (1) all stocks necessary for the production of hybrid and backcross hybrid fishes required in the Program Project are maintained as pedigreed lines at the Stock Center, (2) the Stock Center is the only facility within the two research institutions that has the capacity to house and feed the estimated 2,000 animals that will be bred per year during the course of the estimated 2,000 animals that will be bred per year during the course of the project, and (3) proposed experimental treatments with UV and MNU occur early in the life of the backcross hybrids, and since experimental animals must be maintained for six months or more prior to sacrifice, the Stock Center is the most logical facility for headquartering the core. The specific aims of the Core Project are to ensure that (1) parental stocks required to produce hybrids are maintained in sufficient numbers, (2) all hybridizations produce sufficient F1 progeny for backcross matings, (3) backcross offspring are produced in sufficient numbers for the experimental and control of requirements produced in sufficient numbers for the experimental and control requirements of each project (4) backcross offspring receive the precise experimental treatment, i.e., UV irradiation or MNU dosage specified by each project investigator, (5) the health of the entire animal colony is never compromised, and (6) the genetic backgrounds and stock purity of pedigrees used in these experiments are well characterized. Baseline data regarding the genetic backgrounds of parental and F1 hybrid stocks used to manufacture backcross hybrids are necessary, particularly in light of recent findings of polyallelism in isogenic clonal zebrafish stock which suggest that assumptions of stock homogeneity must also be validated in Xiphophorus stocks. Biochemical genetic profiles for each of the stocks will identify candidates for additional hybridization mating required by each component project to continue their linkage map saturation and/or associated tumor marker mapping efforts. Core facility personnel will screen parents of all broods utilized in the Program for more than 50 protein-coding loci after each mating has been terminated and will screen siblings of the animals used in the Program Project. The significance of establishing a core facility for the Program Project is that it will centralize stock management, parental, hybrid, and backcross animal product, experimental treatment, animal core, and stock purity, thereby streamlining the logistics of the Program Project and substantially decreasing the cost of research.

描述：(申请人描述)位于西南得克萨斯州立大学的剑鱼遗传种群中心将作为该项目的核心设施，因为(1)该项目中生产杂交和回交杂交鱼所需的所有种群都作为系谱系保存在该种群中心，(2)该种群中心是这两个研究机构中唯一有能力在该项目期间每年饲养约2,000只动物期间饲养和饲养估计2,000只动物的设施，(3)建议的UV和MNU实验处理在回交杂交种的生命早期进行，由于实验动物必须在牺牲前保持6个月或更长时间，因此股票中心是总部最合理的核心设施。核心项目的具体目标是确保(1)产生杂交所需的亲本种群保持足够的数量，(2)所有杂交产生足够的F1后代用于回交配对，(3)回交后代的数量足够用于实验和控制每个项目的实验和控制要求(4)回交后代接受精确的实验处理，即每个项目调查员指定的紫外线照射或MNU剂量，(5)不损害整个动物群体的健康，以及(6)这些实验所用家系的遗传背景和种群纯度都有很好的特征。关于用于制造回交杂交种的亲本和F1杂交群体的遗传背景的基线数据是必要的，特别是考虑到最近关于同基因克隆斑马鱼群体的多等位基因的发现，这表明群体同质性的假设也必须在剑鱼群体中得到验证。每个种群的生化遗传图谱将确定每个组成部分项目所需的额外杂交交配的候选对象，以继续其连锁图谱饱和和/或相关的肿瘤标记物作图工作。核心设施人员将在每次交配终止后对计划中使用的所有幼崽的父母进行50多个蛋白质编码基因座的筛选，并将对计划项目中使用的动物的兄弟姐妹进行筛查。为计划项目建立核心设施的意义在于，它将集中管理牲畜、亲本、杂交和回交动物产品、实验处理、动物核心和牲畜纯度，从而简化计划项目的物流并大幅降低研究成本。