CORE--IMMUNOLOGY

免疫学核心旨在提供免疫试剂 在项目1到4中需要。这些试剂是建立的：单克隆 抗人结直肠癌(CRC)抗原抗体(MAb) GA733和小鼠同系物mEGP。针对这些抗原的多克隆抗体将 经Ag免疫亲和层析柱纯化。新的单抗用于片断 将产生GA733银(源自项目1)。来自中国的抗体 (在项目3下)为患者、健康人和患者接种疫苗 对于溃疡性结肠炎，将对详细组织进行亲和纯化 结合特异性分析。此外，核心B将生产提纯的 杆状病毒来源的GA733抗原和mEGP。一批临床级 杆状病毒衍生的GA733抗原将用于患者的皮肤测试 在项目3中，将测试所有试剂的纯度、特性和 免疫反应性。 由经验丰富的调查人员提供的集中服务将 提高抗原和抗体制剂质量，全面节约成本 与将其纳入每个项目的成本进行比较。

The immunology Core is Designed to provide immunologic reagents needed in Project 1 through 4. These reagents are established: monoclonal antibodies (Mabs) to the human colorectal carcinoma (CRC) antigen (Ag) GA733 and the murine homolog mEGP. Polyclonal antibodies to these Ags will be purified on Ag immunoaffinity columns. New Mab to fragments of the GA733 Ag (derived from Project 1) will be generated. Antibodies from vaccinating (under Project 3) patients, healthy individuals and patients with ulcerative colitis, will be affinity-purified for detailed tissue binding specificity analysis. In addition, Core B will produce purified baculovirus-derived GA733 Ag and mEGP. A clinical grade batch of baculovirus-derived GA733 Ag will be produced for skin testing of patients in Project 3. All reagants will be tested for purity, identity, and immunoreactivity. The centralized services provided by experienced investigators will improve quality of the Ag and antibody preparations and save overall costs as compared to their inclusion as part of each project.