RUTHENIUM (II) COMPLEXES AS PROBES OF INTRACELLULAR (INTRA ENDOCYTIC) OXYGEN

钌 (II) 配合物作为细胞内（内吞）氧的探针

• 批准号: 6315374
• 负责人: HM SWARTZ
• 金额: $ 2.83万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6315374
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; microscopy; spectrometry; technology /technique

We measured intra- and extra-cellular concentrations of oxygen in Mouse Monocyte-Macrophage cultures, J774A.1, using the Ru(II) complexes [Ru(bpy)3]2+ and [Ru(phen)3]2+with the fluorescence lifetime microscope. Using the time-resolved technique we were able to compare response for oxygen concentrations between air and nitrogen atmosphere. The oxygen-dependent changes in phase angle for [Ru(bpy)3]2+ and [Ru(phen)3]2+ inside and outside cells were in the order of 10-12 degrees (400 kHz) between the air and nitrogen. The spatial resolution under the microscope was at the diffraction limit, which allowed us to measure the concentration of oxygen inside a single 12 micrometer size macrophage.

我们测量了细胞内和细胞外的氧气浓度 小鼠单核细胞-巨噬细胞培养物，J774A.1，使用 Ru(II) 配合物 [Ru(bpy)3]2+ 和 [Ru(phen)3]2+ 与荧光寿命 显微镜。 使用时间分辨技术我们能够比较 response for oxygen concentrations between air and nitrogen 气氛。 相角的氧依赖性变化 细胞内外的[Ru(bpy)3]2+和[Ru(phen)3]2+分别位于 空气和氮气之间的温度差为 10-12 度 (400 kHz)。 这 显微镜下的空间分辨率达到衍射极限， 这使我们能够测量内部的氧气浓度 单个 12 微米大小的巨噬细胞。