Dissection of a calcium signaling pathway in C. elegans

钙信号在许多细胞过程中起着关键作用，包括造血细胞的增殖和分化、肌肉收缩、肌动蛋白丝解聚和长时程增强。细胞内钙动力学的一个重要组成部分是通过肌醇1，4，5-三磷酸受体（IP3R）释放细胞内钙储存。秀丽隐杆线虫（C.在哺乳动物（如线虫）中，IP3R调节行为节律-后体壁肌肉的周期性收缩。肠道以非自主的方式控制上覆肌肉的收缩，并且肠道钙振荡直接先于肌肉收缩。一种组织（肠）中IP3R的活性如何调节另一种组织（肌肉）的活性尚不清楚。该建议旨在揭示线虫中由IP3R功能启动的快速，新颖的信号级联的分子和细胞基础。将实现以下目标。具体目标1：钙峰在诱导后体收缩中的充分性和必要性具体目标2：确定肠中钙动力学的空间和时间特征具体目标3：确定指导后体收缩的信号的分子基础。.

Calcium signaling plays a key role in many cellular processes including proliferation and differentiation of hematopoietic cells, muscle contraction, actin filament disassembly and long term potentiation. One essential component of intracellular calcium dynamics is release of intracellular calcium stores via inositol 1,4,5-trisphosphate receptors (IP3Rs). In the nematode Caenorhabditis elegans (C. elegans), the IP3R regulates a behavioral rhythm - the periodic contraction of the posterior body wall muscles. The intestine controls the contraction of the overlying muscles in a nonautonomous fashion and intestinal calcium oscillations directly precede the muscle contraction. How the activity of the IP3R within one tissue, the intestine, modulates the activity of another tissue, the muscle, is unknown. This proposal aims to uncover the molecular and cellular basis of the fast, novel signaling cascade initiated by IP3R function in the nematode Caenorhabditis elegans. The following Aims will be addressed. Specific Aim 1: Sufficiency and necessity of calcium spike in induction of the posterior body contraction Specific Aim 2: Determination of the spatial and temporal characteristics of calcium dynamics in the intestine Specific Aim 3: Determination of the molecular basis of the signal that directs posterior body contraction . .