RAF-1 REGULATION BY THE POST-TRANSLATIONAL MOD. O-GLCNAC
RAF-1 REGULATION BY THE POST-TRANSLATIONAL MOD. O-GLCNAC
RAF-1 由翻译后模块调节。
批准号：
6385183
负责人：
KEITH VOSSELLER
金额：
$4.38万
依托单位：
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
项目状态：
未结题
起止时间：
2001-09-01 至
关键词：
G protein N acetylglucosamine N acylation binding sites biological signal transduction cell line enzyme induction /repression enzyme mechanism glycoprotein structure glycoproteins immunoprecipitation intracellular transport mass spectrometry phosphorylation posttranslational modifications protein kinase protein localization protein protein interaction protein structure function protein transport protooncogene site directed mutagenesis
中文摘要
翻译
在细胞质和核蛋白的丝氨酸和苏氨酸上添加O-GlcNAc是一种独特的碳水化合物修饰，没有已知的功能。这一提议提出了O-GlcNAc作为一种控制蛋白质功能状态的动态开关机制的潜力（类似于信号转导中的调节磷酸化）。在信号传导的背景下，O-GlcNAc在调节Raf-1的各个功能方面的作用将被研究。O-GlcNAc以基础或诱导方式调节激酶活性或Raf-1与Ras和14-3-3等关键蛋白相互作用的潜力将被研究。
英文摘要
O-GlcNAc addition to serines and threonines of cytosolic and nuclear proteins is a unique carbohydrate modification with no known function. This proposal addresses the potential for O-GlcNAc to be a dynamic switch mechanism controlling the functional states of proteins (analogous to regulated phosphorylation in signal transduction). In the context of signaling, the role of O-GlcNAc in regulating various functional aspects of Raf-1 will be examined. The potential for O-GlcNAc to regulate kinase activity or interaction of Raf-1 with key proteins such as Ras and 14-3-3 in a basal or inducible way will be studied.
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会议论文
Reversal of Tau Alzheimer's hyperphosphorylation by pharmacological elevation of
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批准号：7769497
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项目类别：
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资助金额：$15.07万
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财政年份：2009
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负责人：KEITH VOSSELLER
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依托单位：
RAF1 REGULATION BY O-GLCNAC MODIFICATION
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批准号：6138341
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项目类别：
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资助金额：$3.92万
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财政年份：2000
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负责人：KEITH VOSSELLER
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依托单位：
海外基金
