Determining how Aly links splicing to mRNA export

确定 Aly 如何将剪接与 mRNA 输出联系起来

• 批准号: 6406363
• 负责人: DENISE M DORMAN
• 金额: $ 3.33万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6406363
• 关键词: RNA binding protein; RNA splicing; Xenopus oocyte; gene deletion mutation; intracellular transport; messenger RNA; precursor mRNA; protein structure function; spliceosomes

The long-term objective of this proposal is to achieve a detailed understanding of the coupling between pre-mRNA splicing and mRNA export. The protein Aly, which is recruited to mRNA during spliceosome assembly and specifically promotes the export of spliced mRNA, is likely to play a key role in linking splicing to mRNA export. Thus, a detailed understanding of this protein and its interactions with these two major nuclear machineries is essential for understanding gene expression. The first specific aim is to map the functional domains in Aly that are required for interactions with the spliceosome and the export machinery. The second specific aim is to determine how Aly is recruited to the mRNA during spliceosome assembly. Finally, the third specific aim is to determine how Aly is targeted to the export machinery. Together, the proposed studies should lead to important insights into the coupling between splicing and mRNA export as well as into the mechanism of mRNA export.

该建议的长期目标是实现对mRNA前剪接和mRNA输出之间耦合的详细理解。在剪接体组装过程中被招募到mRNA并特异性地促进剪接mRNA的输出的蛋白质Aly可能在剪接与mRNA输出的联系中发挥关键作用。因此，详细了解这种蛋白质及其与这两种主要核机制的相互作用对于理解基因表达至关重要。第一个具体目标是绘制Aly中与剪接体和输出机制相互作用所需的功能域。第二个具体目标是确定在剪接体组装过程中Aly是如何被招募到mRNA上的。最后，第三个具体目标是确定Aly如何针对出口机械。综上所述，这些研究将有助于了解剪接与mRNA输出之间的耦合以及mRNA输出的机制。