MALENESS IN DROSOPHILA MELANOGASTER

果蝇的雄性特征

• 批准号: 6298630
• 负责人: TROY CARLO
• 金额: $ 4.02万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6298630
• 关键词: Drosophilidae; behavioral genetics; developmental genetics; fertility; gene mutation; genetic mapping; male; postdoctoral investigator; sex behavior; sex determination; suppressor mutations

The goal of this proposal is to understand the genes required for maleness in Drosophila melanogaster. The first set of studies is directed at finding additional genes in the fruitless (fru) branch of the sex-determination pathway. This branch has been implicated in the development and/or maintenance of male specific courtship behavior as well as the development of male specific Muscle of Lawrence (MOL). Using correct MOL development as a visual tool, various genes will be expressed in the nerves that are critical for MOL development. If the expression of a gene in MOL neurons, in a fru mutant background, rescues MOL development then that gene will be considered as part of the fru pathway. IN addition, a genetic screen will be performed to identify suppressor mutations of the original fru1 mutation. The second set of experiments is directed at understanding cues males us to identify potential mates. A locus, fruitless stimulation factor (fsf), when mutated gives rise a mutant phenotype of males which are vigorously courted by other males. Normal males as a rule are not strongly courted by other normal males. Current studies have pinpointed the region that harbors the mutation. Database analysis has demonstrated that two transcribed regions or separate sets of EST's are located very near the mutation. Future experiments will be directed at determining which of t he two potential transcription units if the gene referred to as fsf as well as the role of the gene in the establishment of maleness.

这项计划的目标是了解果蝇雄性所需的基因。第一组研究是在性别决定途径的无果（fru）分支中寻找额外的基因。该分支与雄性特有求偶行为的发育和/或维持以及雄性特有劳伦斯肌（MOL）的发育有关。使用正确的MOL发育作为视觉工具，各种基因将在对MOL发育至关重要的神经中表达。如果在fru突变背景下，MOL神经元中的基因表达挽救了MOL发育，则该基因将被视为fru途径的一部分。此外，将进行遗传筛选以鉴定原始fru 1突变的抑制突变。第二组实验旨在了解男性识别潜在伴侣的线索。一个基因座，无果刺激因子（fsf），当突变时产生雄性的突变表型，这些表型被其他雄性强烈地追求。正常的雄性通常不会受到其他正常雄性的强烈追求。目前的研究已经确定了携带突变的区域。数据库分析表明，两个转录区域或独立的EST组位于非常接近的突变。未来的实验将致力于确定这两个潜在的转录单位中的哪一个，如果该基因被称为fsf，以及该基因在建立雄性中的作用。