GDF-8 (MYOSTATIN) AND GDF-11

描述(改编自调查人员摘要)：这项建议是 关注被分离的转化生长因子-b超家族的两个成员 调查员、GDF-8和GDF-11。GDF-8几乎只在 发育中和成年的骨骼肌和零突变小鼠表现出巨大的和 骨骼肌生长普遍增加。有六个目标 研究GDF-8和GDF-11的生物学功能。第一个目标是 为了在RNA和蛋白质水平上表征基因的表达模式 GDF-8和GDF-11。对于GDF-8转录本，这一点已经实现。 第二个目的是鉴定GDF-8和GDF-11的表型 零老鼠。第三个具体目标是从哺乳动物中提纯蛋白质。 细胞，并确定各种加工形式之间的相互作用。 第四个目标是确定GDF-8和GDF-11的分布 感受器。第五个目的是测试GDF-8和GDF-11的功能 蛋白质，以验证GDF-8作为一种 成肌细胞增殖的抑制因子。最终的目标是克隆这些基因 GDF-8和GDF-11受体。

DESCRIPTION (adapted from investigator's abstract): This proposal is concerned with two members of the TGF-b superfamily isolated by the investigator, GDF-8 and GDF-11. GDF-8 is expressed almost exclusively in developing and adult skeletal muscle and null mutant mice show a massive and widespread increase in skeletal muscle growth. There are six aims to investigate the biological functions of GDF-8 and GDF-11. The first aim is to characterize the expression patterns at the RNA and protein level of GDF-8 and GDF-11. This has already been achieved for GDF-8 transcripts. The second aim is to characterize the phenotypes of the GDF-8 and GDF-11 null mice. The third specific aim is to purify the proteins from mammalian cells and determine the interaction between the various processed forms. The fourth aim is to determine the distribution of the GDF-8 and GDF-11 receptors. The fifth aim is to test the function of GDF-8 and GDF-11 protein on cultured cells to test the hypothesis that GDF-8 acts as an inhibitor of myoblast proliferation. The final aim is to clone the genes for the GDF-8 and GDF-11 receptors.