MODULATION OF OLFACTORY BULB NEURON CURRENT PROPERTIES

嗅球神经元电流特性的调节

• 批准号: 6342339
• 负责人: DEBRA Ann FADOOL
• 金额: $ 11.29万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6342339
• 关键词: biological signal transduction; cell line; electrophysiology; enzyme activity; laboratory rat; membrane channels; neural plasticity; neurons; olfactory lobe; phosphorylation; potassium channel; protein tyrosine kinase; site directed mutagenesis; tissue /cell culture; voltage gated channel

(Adapted from the Investigator's Abstract): The designed research is a multidisciplinary analysis of the modulation of the potassium currents in granule and mitral cells of the olfactory bulb. The broad, long-term objective of this research is to elucidate how neurotropins, growth factors, and cytoplasmic protein kinases can utilize ion channels as substrates for phosphorylation to give rise to short-term and long-term plastic changes in synaptic efficacy or to aid in the establishment of neural circuits in the olfactory bulb neurons, Kvl.3, using electrophysiological, biochemical, and molecular approaches. We will electrophysiologically examine changes in current magnitude and gating kinetics induced by activating tyrosine phosphorylation in granule and mitral cells and measure the degree of tyrosine specific phosphorylation. By utilizing the cloned Kvl.3 as a model, combined biochemical measurement of kinase-induced tyrosine phosphorylation and molecular mutagenesis will elucidate the mechanistic details of multiple phosphorylation of the ion channel. The proposal will provide new important information regarding the molecular mechanism for the convergence of multiple phosphorylation on an ion channel prominently expressed in the olfactory bulb, a brain region for which neuromodulation by phosphorylation is completely undescribed.

（改编自研究者摘要）：设计的研究是一个 钾电流调制的多学科分析， 嗅球的颗粒细胞和僧帽细胞。广泛、长期 本研究的目的是阐明神经营养素，生长， 细胞因子和细胞质蛋白激酶可以利用离子通道， 磷酸化的底物，以产生短期和长期的 可塑性的变化，突触的功效或帮助建立 嗅球神经元中的神经回路，Kvl.3，使用 电生理学、生物化学和分子方法。我们将 电生理学检查电流大小和门控的变化 激活颗粒中酪氨酸磷酸化诱导的动力学， 并测量酪氨酸特异性磷酸化的程度。 通过利用克隆的Kvl.3作为模型，结合生物化学测量， 激酶诱导的酪氨酸磷酸化和分子诱变将 阐明离子的多重磷酸化的机制细节 频道该提案将提供有关以下方面的新的重要信息： 多磷酸化聚合的分子机制 离子通道在嗅球（大脑区域）中显著表达 其通过磷酸化的神经调节是完全未描述的。