ROLE OF SURFACE DEHYDROGENASE IN STREPTOCCAL INFECTION

表面脱氢酶在链球菌感染中的作用

• 批准号: 6373802
• 负责人: VIJAY PANCHOLI
• 金额: $ 27.23万
• 依托单位: PUBLIC HEALTH RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6373802
• 关键词: Streptococcus pyogenes; bacteria infection mechanism; bacterial proteins; biological signal transduction; cell adhesion; enzyme activity; epithelium; fluorescent dye /probe; gene mutation; glyceraldehyde 3 phosphate dehydrogenase; microorganism metabolism; protein structure function; receptor binding; recombinant proteins; site directed mutagenesis; surface antigens; tissue /cell culture

The PI hypothesizes that a surface dehydrogenase (SDH) plays a critical role in the early events of infection by facilitating entry of group A streptococcus into pharyngeal cells. The author states that SDH is essential for survival of GAS and that SDH has multiple means of binding to several mammalian proteins and also has ADP-ribosylating activity. SDH interacts with mammalian pharyngeal cells and regulates the signal transduction pathways in pharyngeal cells which facilitates adherence and penetration of the host cell. Specific Aim 1. A 32 kDa pharyngeal cell membrane receptor for SDH will be purified and biochemically characterized Specific Aim 2. The receptor binding domain of SDH will be identified using several protein and site directed mutagenesis techniques to establish the molecular basis of SDH-pharyngeal cell receptor interaction. Specific Aim 3 Using intact streptococci and recombinant native and mutated SDH proteins, SDH mediated signaling pathways in pharyngeal cells will be elucidated by studying various intracellular signaling events. Specific Aim 4, native and mutated SDH will be covalently linked to fluorescinated microbeads to study adherence and invasion of cell lines.

PI假设一种表面脱氢酶（SDH）在其中起作用