CHLORIDE CHANNEL OF OSTEOCLAST RUFFLED BORDER
CHLORIDE CHANNEL OF OSTEOCLAST RUFFLED BORDER
批准号:
6375073
负责人:
JOHN C EDWARDS
金额:
$18.37万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-04-01 至 2003-03-31
关键词:
RNase protection assay SDS polyacrylamide gel electrophoresis autoradiography biological transport chloride channels gel filtration chromatography hydrochloric acid immunoprecipitation molecular cloning nucleic acid sequence osteoclasts pathologic bone resorption phosphorylation physiologic bone resorption polymerase chain reaction protein tyrosine kinase stainings tissue /cell culture western blottings
中文摘要
描述(改编自申请人摘要):适当的法规
破骨细胞介导的骨吸收对于维持
健康的骨骼 了解破骨细胞的分子调控,
为常见的严重骨骼疾病带来新的治疗方法,
骨质疏松 破骨细胞通过产生酸性隔室来吸收骨
在骨头表面。 破骨细胞酸运输的关键成分
机制是一个氯离子通道,表达在破骨细胞-皱褶
边境 申请人的长期目标是鉴定
皱褶边缘氯离子通道的基础,并了解这是如何
通道可调节酸转运和骨吸收。 具体目标
本建议的主要内容是:(1)完成
编码皱褶膜氯离子通道的序列;(2)生物化学
(3)探讨了通道复合体与
氯离子通道和非受体酪氨酸激酶,src,和;(4)定义
骨的存在调节骨的表达的机制,
氯离子通道
英文摘要
DESCRIPTION (Adapted from the Applicant's Abstract): Appropriate regulation
of osteoclast-mediated bone resorption is essential for maintenance of
healthy bone. Understanding the molecular regulation of osteoclasts may
lead to new therapeutic approaches to common serious bone diseases like
osteoporosis. Osteoclasts resorb bone by generating an acidic compartment
on the bone surface. A key component of the osteoclast acid transport
mechanism is a chloride channel which is expressed in the osteoclast-ruffled
border. The applicant's long-term goals have been to identify the molecular
basis of the ruffled border chloride channel and to understand how this
channel may regulate acid transport and bone resorption. The Specific Aims
of the present proposal are to: (1) complete molecular cloning of the
sequences encoding the ruffled membrane chloride channel; (2) biochemically
characterize the channel complex; (3) explore the relationship between the
chloride channel and the non-receptor tyrosine kinases, src, and; (4) define
the mechanism by which the presence of bone regulates expression of the
chloride channel.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
ApoL1 and its kidney disease associated variants: ion permease activity, molecular structure, and podocyte injury.
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批准号:9896822
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项目类别:
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资助金额:$37.28万
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财政年份:2019
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负责人:JOHN C EDWARDS
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依托单位:
ApoL1 and its kidney disease associated variants: ion permease activity, molecular structure, and podocyte injury.
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批准号:10371206
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项目类别:
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资助金额:$37.28万
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财政年份:2019
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负责人:JOHN C EDWARDS
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依托单位:
The Role of CLIC4 in Angiogenesis
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批准号:7894662
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项目类别:
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资助金额:$38.23万
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财政年份:2009
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负责人:JOHN C EDWARDS
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依托单位:
The Role of CLIC4 in Angiogenesis
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批准号:8065877
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项目类别:
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资助金额:$0.9万
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财政年份:2009
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负责人:JOHN C EDWARDS
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依托单位:
The Role of CLIC4 in Angiogenesis
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批准号:8282835
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项目类别:
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资助金额:$38.23万
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财政年份:2009
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负责人:JOHN C EDWARDS
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依托单位:
The Role of CLIC4 in Angiogenesis
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批准号:8470213
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项目类别:
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资助金额:$35.88万
-
财政年份:2009
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负责人:JOHN C EDWARDS
-
依托单位:
The Role of CLIC4 in Angiogenesis
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批准号:8326798
-
项目类别:
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资助金额:$36.94万
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财政年份:2009
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负责人:JOHN C EDWARDS
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依托单位:
The Role of CLIC4 in Angiogenesis
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批准号:7730326
-
项目类别:
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资助金额:$44.43万
-
财政年份:2009
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负责人:JOHN C EDWARDS
-
依托单位:
The CLIC-1 Chloride Channel: Structure and Function
-
批准号:6711141
-
项目类别:
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资助金额:$19.87万
-
财政年份:2003
-
负责人:JOHN C EDWARDS
-
依托单位:
The CLIC-1 Chloride Channel: Structure and Function
-
批准号:7010368
-
项目类别:
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资助金额:$23.45万
-
财政年份:2003
-
负责人:JOHN C EDWARDS
-
依托单位:
The CLIC-1 Chloride Channel: Structure and Function
-
批准号:6858651
-
项目类别:
-
资助金额:$15.45万
-
财政年份:2003
-
负责人:JOHN C EDWARDS
-
依托单位:
The CLIC-1 Chloride Channel: Structure and Function
-
批准号:7201603
-
项目类别:
-
资助金额:$22.77万
-
财政年份:2003
-
负责人:JOHN C EDWARDS
-
依托单位:
The CLIC-1 Chloride Channel: Structure and Function
-
批准号:7169345
-
项目类别:
-
资助金额:$4.42万
-
财政年份:2003
-
负责人:JOHN C EDWARDS
-
依托单位:
The CLIC-1 Chloride Channel: Structure and Function
-
批准号:6611726
-
项目类别:
-
资助金额:$22.22万
-
财政年份:2003
-
负责人:JOHN C EDWARDS
-
依托单位:
CHLORIDE CHANNEL OF OSTEOCLAST RUFFLED BORDER
-
批准号:6129911
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项目类别:
-
资助金额:$13.56万
-
财政年份:1998
-
负责人:JOHN C EDWARDS
-
依托单位:
CHLORIDE CHANNEL OF OSTEOCLAST RUFFLED BORDER
-
批准号:2448844
-
项目类别:
-
资助金额:$21.83万
-
财政年份:1998
-
负责人:JOHN C EDWARDS
-
依托单位:
CHLORIDE CHANNEL OF OSTEOCLAST RUFFLED BORDER
-
批准号:6171372
-
项目类别:
-
资助金额:$17.83万
-
财政年份:1998
-
负责人:JOHN C EDWARDS
-
依托单位:
CHLORIDE CHANNEL OF OSTEOCLAST RUFFLED BORDER
-
批准号:2899924
-
项目类别:
-
资助金额:$6.63万
-
财政年份:1998
-
负责人:JOHN C EDWARDS
-
依托单位:
MOLECULAR CHARACTERIZATION OF CHLORIDE CHANNEL PROTEINS
-
批准号:2518339
-
项目类别:
-
资助金额:$10.13万
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财政年份:1993
-
负责人:JOHN C EDWARDS
-
依托单位:
MOLECULAR CHARACTERIZATION OF CHLORIDE CHANNEL PROTEINS
-
批准号:2145401
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项目类别:
-
资助金额:$9.73万
-
财政年份:1993
-
负责人:JOHN C EDWARDS
-
依托单位: